Single-Neuron Level One-Photon Voltage Imaging With Sparsely Targeted Genetically Encoded Voltage Indicators

Frontiers in Cellular Neuroscience
Peter QuickeThomas Knöpfel


Voltage imaging of many neurons simultaneously at single-cell resolution is hampered by the difficulty of detecting small voltage signals from overlapping neuronal processes in neural tissue. Recent advances in genetically encoded voltage indicator (GEVI) imaging have shown single-cell resolution optical voltage recordings in intact tissue through imaging naturally sparse cell classes, sparse viral expression, soma restricted expression, advanced optical systems, or a combination of these. Widespread sparse and strong transgenic GEVI expression would enable straightforward optical access to a densely occurring cell type, such as cortical pyramidal cells. Here we demonstrate that a recently described sparse transgenic expression strategy can enable single-cell resolution voltage imaging of cortical pyramidal cells in intact brain tissue without restricting expression to the soma. We also quantify the functional crosstalk in brain tissue and discuss optimal imaging rates to inform future GEVI experimental design.


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Methods Mentioned

fluorescence imaging
confocal microscopy
fluorescence microscopy
fluorescence collection

Related Concepts

Cell Separation
Diagnostic Imaging
Plants, Transgenic
Voltage-Clamp Techniques
Brain Tissue
Intercellular Communication Process
Primary Sex Determination, Soma
Cell Cortex

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