Single-nuclei RNA-seq on human retinal tissue provides improved transcriptome profiling.

BioRxiv : the Preprint Server for Biology
Qingnan LiangRui Chen

Abstract

The retina is the innermost layer of tissue in the eyes of human and most other vertebrates. It receives the information of the visual images like the film of a camera, translates the images into neural signals, and transduces the signal to the brain. Three layers of neural cells (photoreceptor cells, bipolar cells, and ganglion cells) within the retina are comprised of seven major cell types; rod, cone, Muller glia cell, amacrine cell, horizontal cell, bipolar cell, and retinal ganglion cells, which create visual perception through functional cooperation. In all retinal diseases, it is the ultimate degeneration of the photoreceptors, the rods and cones, which results in blindness. Understanding all the individual cell types and how they contribute the basic neural circuitry in both the human and mouse retina will be key to elucidating the mechanism of retinal degenerations. Here, we report to the best of our knowledge the first single-nuclei RNA-seq based transcriptomic study on the human neural retinal tissue. We sequenced 6544 nuclei from six samples, and 4730 nuclei passed the quality filtering steps. The donor samples came from both the macular and peripheral regions, respectively, from three donor's retina. None of the do...Continue Reading

Related Concepts

Biological Markers
Brain
Cell Nucleus
Donor Person
Eye
Gene Expression
Genes
Laboratory mice
Nerve Degeneration
Neuroglia

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