In the present genomics era, powerful reverse-genetic strategies are needed to elucidate gene and protein function in the context of a whole organism. However, most current techniques lack the generality and high-throughput potential of descriptive genomic approaches, such as those that rely on microarray hybridization. For example, in plant research, effective insertional mutagenesis and transgenic methods are limited to relatively few species or are inefficient. Fortunately, single-nucleotide changes can be induced in any plant by using traditional chemical mutagens, and progress has been made in efficiently detecting changes. Because base substitutions in proteins provide allelic series, and not just knockouts, this strategy can yield refined insights into protein function. Here, we review recent progress that has been made in genome-wide screening for point mutations and natural variation in plants. Its general applicability leads to the expectation that traditional mutagenesis followed by high-throughput detection will become increasingly important for plant functional genomics.
Continuous, on-line DNA sequencing using a versatile infrared laser scanner/electrophoresis apparatus
The Adh gene promoters of Drosophila melanogaster and Drosophila orena are functionally conserved and share features of sequence structure and nuclease-protected sites.
EMS- and radiation-induced mutation frequencies at individual loci in Arabidopsis thaliana (L.) Heynh
PolyPhred: automating the detection and genotyping of single nucleotide substitutions using fluorescence-based resequencing
Detection of numerous Y chromosome biallelic polymorphisms by denaturing high-performance liquid chromatography
Virus resistance and gene silencing in plants can be induced by simultaneous expression of sense and antisense RNA
An exploration of the sequence of a 2.9-Mb region of the genome of Drosophila melanogaster: the Adh region.
Genetic repair of mutations in plant cell-free extracts directed by specific chimeric oligonucleotides
A simple procedure for the analysis of single nucleotide polymorphisms facilitates map-based cloning in Arabidopsis
High-throughput multiplex SNP genotyping with MALDI-TOF mass spectrometry: practice, problems and promise
Temperature sensitivity in peroxisome assembly processes characterizes milder forms of peroxisome biogenesis disorders
T-DNA-associated duplication/translocations in Arabidopsis. Implications for mutant analysis and functional genomics
Allele-specific CAPS markers based on point mutations in resistance alleles at the pvr1 locus encoding eIF4E in Capsicum
A single-base substitution suppresses flower color mutation caused by a novel miniature inverted-repeat transposable element in gentian
Analytical methods for inferring functional effects of single base pair substitutions in human cancers.
Predicting the effects of coding non-synonymous variants on protein function using the SIFT algorithm
TILLING in Lotus japonicus identified large allelic series for symbiosis genes and revealed a bias in functionally defective ethyl methanesulfonate alleles toward glycine replacements
Development and characterization of an oat TILLING-population and identification of mutations in lignin and beta-glucan biosynthesis genes
A high-throughput virus-induced gene silencing protocol identifies genes involved in multi-stress tolerance
Simultaneous mutation detection of three homoeologous genes in wheat by High Resolution Melting analysis and Mutation Surveyor
In vitro vs in silico detected SNPs for the development of a genotyping array: what can we learn from a non-model species?
High-throughput discovery of chloroplast and mitochondrial DNA polymorphisms in Brassicaceae species by ORG-EcoTILLING
High-throughput sequencing and mutagenesis to accelerate the domestication of Microlaena stipoides as a new food crop
Investigation of intercellular salicylic acid accumulation during compatible and incompatible Arabidopsis-pseudomonas syringae interactions using a fast neutron-generated mutant allele of EDS5 identified by genetic mapping and whole-genome sequencing
EMS mutagenesis and qPCR-HRM prescreening for point mutations in an embryogenic cell suspension of grapevine
EMS mutagenesis in mature seed-derived rice calli as a new method for rapidly obtaining TILLING mutant populations
Metabolomic and transcriptomic analysis of the rice response to the bacterial blight pathogen Xanthomonas oryzae pv. oryzae.
Spontaneous and induced variability of allergens in commodity crops: Ara h 2 in peanut as a case study
Plant cell wall biosynthesis: genetic, biochemical and functional genomics approaches to the identification of key genes
CZI Human Cell Atlas Seed Network
The aim of the Human Cell Atlas (HCA) is to build reference maps of all human cells in order to enhance our understanding of health and disease. The Seed Networks for the HCA project aims to bring together collaborators with different areas of expertise in order to facilitate the development of the HCA. Find the latest research from members of the HCA Seed Networks here.