Single-nucleotide polymorphism characterization of gametocyte development 1 gene in Plasmodium falciparum isolates from Baringo, Uasin Gishu, and Nandi Counties, Kenya

Heliyon
Josephat K BungeiSteven G Nyanjom

Abstract

Plasmodium falciparum relies on gametocytogenesis to transmit from humans to mosquitoes. Gametocyte development 1 (Pfgdv1) is an upstream activator and epigenetic controller of gametocytogenesis. The emergence of drug resistance is a major public health concern and this requires the development of new strategies that target the transmission of malaria. As a putative drug target, Pfgdv1 has not been characterized to identify its polymorphisms and alleles under selection and how such polymorphisms influence protein structure. This study characterized single-nucleotide polymorphisms (SNPs) in primary sequences (n = 30) of Pfgdv1 gene generated from thirty blood samples collected from patients infected with P. falciparum and secondary sequences (n = 216) retrieved from PlasmoDB. ChromasPro, MUSCLE, Tajima's D statistic, SLAC, and STRUM were used in editing raw sequences, performing multiple sequence alignment (MSA), identifying signatures of selection, detecting codon sites under selection pressure, and determining the effect of SNPs, respectively. MSA of primary and secondary sequences established the existence of five SNPs, consisting of four non-synonymous substitutions (nsSNPs) (p.P217H, p.R398Q, p.H417N, and p.D497E), and a sy...Continue Reading

References

Oct 9, 2002·Nature·Malcolm J GardnerBart Barrell
Feb 11, 2005·Molecular Biology and Evolution·Sergei L Kosakovsky Pond, Simon D W Frost
May 6, 2005·Journal of Clinical Microbiology·Kathy A MangoldKaren L Kaul
May 9, 2007·Nucleic Acids Research·Andreas UntergasserJack A M Leunissen
Oct 30, 2008·Nucleic Acids Research·Cristina AurrecoecheaHaiming Wang
Mar 19, 2014·Journal of Internal Medicine·C Arama, M Troye-Blomberg
Aug 15, 2014·Cell Host & Microbe·Nicolas M B BrancucciTill S Voss
Apr 22, 2016·Malaria Journal·Susana CampinoTaane G Clark
Apr 9, 2017·Scientific Reports·Sherwin ChanJessada Thutkawkorapin
Apr 30, 2017·International Journal for Parasitology·D P Bechtsi, A P Waters
May 4, 2018·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura
May 13, 2018·Trends in Parasitology·Edward ReaRita Tewari
Aug 8, 2018·Trends in Parasitology·M J DelvesA M Blagborough

❮ Previous
Next ❯

Datasets Mentioned

BETA
MN986482-MN986511

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

MUSCLE
MEGA X
STRUM
MSA
PlasmoDB
Sequence Manipulation Suite
Primer3Plus
nsSNP
multifasta
SLAC

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.