Single nucleotide polymorphism detection using gold nanoprobes and bio-microfluidic platform with embedded microlenses

Biotechnology and Bioengineering
Iwona Bernacka-WojcikR Martins

Abstract

The use of microfluidics platforms combined with the optimal optical properties of gold nanoparticles has found plenty of application in molecular biosensing. This paper describes a bio-microfluidic platform coupled to a non-cross-linking colorimetric gold nanoprobe assay to detect a single nucleotide polymorphism associated with increased risk of obesity fat-mass and obesity-associated (FTO) rs9939609 (Carlos et al., 2014). The system enabled significant discrimination between positive and negative assays using a target DNA concentration of 5 ng/µL below the limit of detection of the conventionally used microplate reader (i.e., 15 ng/µL) with 10 times lower solution volume (i.e., 3 µL). A set of optimization of our previously reported bio-microfluidic platform (Bernacka-Wojcik et al., 2013) resulted in a 160% improvement of colorimetric analysis results. Incorporation of planar microlenses increased 6 times signal-to-loss ratio reaching the output optical fiber improving by 34% the colorimetric analysis of gold nanoparticles, while the implementation of an optoelectronic acquisition system yielded increased accuracy and reduced noise. The microfluidic chip was also integrated with a miniature fiber spectrometer to analyze the ...Continue Reading

References

Oct 17, 1998·Science·M A BurnsD T Burke
Aug 3, 2002·Applied Optics·Takeo TanaamiHideyuki Ishida
Dec 6, 2005·Biotechnology Advances·Chunsun ZhangWenling Zheng
Apr 29, 2006·Electrophoresis·Pertti J Viskari, James P Landers
Oct 28, 2009·Biosensors & Bioelectronics·Iwona Bernacka-WojcikRodrigo Martins
Feb 6, 2010·Colloids and Surfaces. B, Biointerfaces·Gonçalo DoriaPedro V Baptista
Feb 25, 2010·Chemical Society Reviews·Daniel MarkRoland Zengerle
Feb 26, 2010·Journal of Nanobiotechnology·João CondePedro V Baptista
Oct 12, 2010·Biosensors & Bioelectronics·Khashayar KhoshmaneshKourosh Kalantar-zadeh
Oct 18, 2011·Journal of Micromechanics and Microengineering : Structures, Devices, and Systems·A Tripathi, N Chronis
Nov 29, 2011·Journal of Proteomics·Miguel Larguinho, Pedro V Baptista
Mar 23, 2012·Sensors·Gonçalo DoriaPedro V Baptista
Sep 25, 2012·Lab on a Chip·Bruno VeigasPedro Viana Baptista
May 11, 2013·Biosensors & Bioelectronics·Iwona Bernacka-WojcikRodrigo Martins
Jul 23, 2013·Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy·Fábio Ferreira CarlosPedro Viana Baptista
Aug 1, 2014·Analytical Biochemistry·Fábio Ferreira CarlosPedro Viana Baptista
Jan 1, 2011·Microfluidics and Nanofluidics·Seokheun ChoiJunseok Chae

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Citations

Aug 12, 2015·Colloids and Surfaces. B, Biointerfaces·Prayanka RajendranLászló Demkó
Nov 24, 2016·Diagnostics·Mílton CordeiroPedro Viana Baptista
Jul 5, 2017·Sensors·Beatriz CoelhoPedro V Baptista
Aug 2, 2020·Biosensors & Bioelectronics·Yong ZhaoYun Peng

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