Single-nucleotide repeat analysis for subtyping Bacillus anthracis isolates

Journal of Clinical Microbiology
Chad W StratiloDoug Bader

Abstract

Single-nucleotide repeats (SNRs) are variable-number tandem repeats that display very high mutation rates. In an outbreak situation, the use of a marker system that exploits regions with very high mutation rates, such as SNRs, allows the differentiation of isolates with extremely low levels of genetic diversity. This report describes the identification and analysis of SNR loci of Bacillus anthracis. SNR loci were selected in silico, and the loci with the highest diversity were used to design and test locus-specific primers against a number of B. anthracis strains with the same multilocus variable-number tandem repeat analysis (MLVA) genotype. SNR markers that allowed strains with the same MLVA genotype to be differentiated from each other were identified. The resulting SNR marker system can be used as a molecular epidemiological tool in a natural outbreak or bioterrorism event, offering the best chance of distinguishing very closely related isolates.

References

Jul 1, 1995·Journal of Clinical Microbiology·L J HarrellK H Wilson
Jun 10, 1998·Microbiology and Molecular Biology Reviews : MMBR·A van BelkumH Verbrugh
Jul 15, 1998·Current Microbiology·E HelgasonA B Kolstø
Jan 18, 2002·Antimicrobial Agents and Chemotherapy·Amy J VoglerPaul Keim
Apr 7, 2004·Applied and Environmental Microbiology·Eran DiamantYechezkel Kashi
Sep 7, 2004·Proceedings of the National Academy of Sciences of the United States of America·Talima PearsonPaul Keim
Sep 29, 2004·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Paul KeimDavid M Wagner
Jan 12, 2005·Genome Biology·Michael E ZwickAlfred Mateczun

❮ Previous
Next ❯

Citations

Dec 1, 2010·Tropical Animal Health and Production·Gholamreza Moazeni JulaKeyvan Tadayon
Mar 17, 2010·Biosecurity and Bioterrorism : Biodefense Strategy, Practice, and Science·Willy A Valdivia-Granda
Nov 6, 2013·Biosecurity and Bioterrorism : Biodefense Strategy, Practice, and Science·Sylviane Derzelle, Simon Thierry
May 20, 2008·Applied and Environmental Microbiology·Pierre WattiauHein Imberechts
Jul 10, 2012·Applied and Environmental Microbiology·Chad W Stratilo, Douglas E Bader
Nov 28, 2012·BMC Genomics·Sebastián Aguilar PierléKelly A Brayton
Mar 2, 2010·BMC Veterinary Research·Giuliano GarofoloFlorigio Lista
Mar 14, 2012·PLoS Neglected Tropical Diseases·Wolfgang BeyerPeter C B Turnbull
Sep 3, 2009·Molecular Aspects of Medicine·W Beyer, P C B Turnbull
Sep 15, 2009·Veterinary Microbiology·Antonio FasanellaMartin Hugh Jones
Jul 8, 2008·Molecular and Cellular Probes·Markus H AntwerpenHermann Meyer
Sep 24, 2015·Acta Tropica·Norbert Georg SchwarzHagen Frickmann
May 21, 2010·FEMS Immunology and Medical Microbiology·Larisa N IkryannikovaVadim M Govorun
Aug 27, 2015·PloS One·Wei ZhouXuejun Guo
Dec 29, 2012·Journal of Veterinary Science·Kyoung Hwa JungYoung Gyu Chai
Feb 16, 2020·BMC Infectious Diseases·Enmin ZhangJianchun Wei
Jan 18, 2017·Infectious Diseases of Poverty·Dong-Li LiuWei Li
Nov 6, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A van BelkumUNKNOWN European Society of Clinical Microbiology and Infectious Diseases (ESCMID) Study Group on Epidemiological Markers (ESGEM)

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anthrax Vaccines

Three different types of anthrax vaccines are available; a live-attenuated, an alum-precipitated cell-free filtrate and a protein recombinant vaccine. The effectiveness between the three is uncertain, but the live-attenuated have shown to reduce the risk of anthrax with low adverse events. Here is the latest research on anthrax vaccines.

Anthrax

Anthrax toxin, comprising protective antigen, lethal factor, and oedema factor, is the major virulence factor of Bacillus anthracis, an agent that causes high mortality in humans and animals. Here is the latest research on Anthrax.

Anthrax Vaccines (ASM)

Three different types of anthrax vaccines are available; a live-attenuated, an alum-precipitated cell-free filtrate and a protein recombinant vaccine. The effectiveness between the three is uncertain, but the live-attenuated have shown to reduce the risk of anthrax with low adverse events. Here is the latest research on anthrax vaccines.