Single-nucleus RNA-seq and FISH reveal coordinated transcriptional activity in mammalian myofibers

BioRxiv : the Preprint Server for Biology
M. Dos SantosPascal Maire


Skeletal muscle fibers are large syncytia but it is currently unknown whether gene expression is coordinately regulated in their numerous nuclei. By snRNA-seq and snATAC-seq, we showed that slow, fast, myotendinous and neuromuscular junction myonuclei each have different transcriptional programs, associated with distinct chromatin states and combinations of transcription factors. In adult mice, identified myofiber types predominantly express either a slow or one of the three fast isoforms of Myosin heavy chain (MYH) proteins, while a small number of hybrid fibers can express more than one MYH. By snRNA-seq and FISH, we showed that the majority of myonuclei within a myofiber are synchronized, coordinately expressing only one fast Myh isoform with a preferential pane of muscle-specific genes. Importantly, this coordination of expression occurs early during post-natal development and depends on innervation. These findings highlight a unique mechanism of coordination of gene expression in a syncytium.

Related Concepts

Cell Communication
Prostate Carcinoma
Secondary Malignant Neoplasm of Bone
Cancer Cell Growth
Bone Matrix
Division of Basic Sciences
Positioning Patient (Procedure)

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

Adenoma, Liver Cell

Liver Cell Adenoma or hepatic adenoma is a rare benign tumor. It is associated with birth control use or pregnancy. Discover the latest research on Liver Cell Adenoma here.

Related Papers

European Journal of Orthodontics
Anna M ÖdmanMark P Lewis
Annual Review of Cell and Developmental Biology
A Weiss, L Leinwand
Journal of Cancer Research and Clinical Oncology
Eyun-Jung ShinBharati Bapat
Proceedings of the National Academy of Sciences of the United States of America
S J YoonL Leinwand
© 2020 Meta ULC. All rights reserved