Single-particle cryo-EM-How did it get here and where will it go

Science
Yifan Cheng

Abstract

Cryo-electron microscopy, or simply cryo-EM, refers mainly to three very different yet closely related techniques: electron crystallography, single-particle cryo-EM, and electron cryotomography. In the past few years, single-particle cryo-EM in particular has triggered a revolution in structural biology and has become a newly dominant discipline. This Review examines the fascinating story of its start and evolution over the past 40-plus years, delves into how and why the recent technological advances have been so groundbreaking, and briefly considers where the technique may be headed in the future.

References

Jan 1, 1978·Ultramicroscopy·J FrankT S Baker
May 25, 1975·Journal of Molecular Biology·P N Unwin, R Henderson
Jun 20, 1990·Journal of Molecular Biology·R HendersonK H Downing
May 1, 1988·Quarterly Reviews of Biophysics·J DubochetP Schultz
Dec 13, 1974·Science·K A Taylor, R M Glaeser
Mar 1, 1984·Nature·M AdrianA W McDowall
Feb 3, 1995·Journal of Molecular Biology·S SrivastavaJ Frank
Oct 17, 1998·Journal of Structural Biology·F J Sigworth
Dec 4, 2003·Journal of Structural Biology·Steven J Ludtke, Wah Chiu
Dec 5, 2003·Nature·David E Clapham
May 14, 2005·Journal of Structural Biology·Christian SulowayBridget Carragher
Mar 1, 2007·Methods in Cell Biology·Paul Mooney
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Melanie D OhiThomas Walz
Feb 4, 2011·Advances in Experimental Medicine and Biology·Minghui LiJian Yang
Nov 22, 2011·Journal of Molecular Biology·Sjors H W Scheres
Jan 13, 2012·Nature·Gabriel C LanderAndreas Martin
Mar 1, 2012·Journal of Structural Biology·Axel F BrilotNikolaus Grigorieff
Sep 25, 2012·Journal of Structural Biology·Sjors H W Scheres
Aug 24, 2013·Journal of Structural Biology·Xueming LiYifan Cheng
Oct 9, 2013·Annual Review of Cell and Developmental Biology·David Julius
Jan 31, 2014·Nature·Nicola Jones
Feb 1, 2014·Current Opinion in Structural Biology·Wojciech P GalejKiyoshi Nagai
Oct 22, 2014·Proceedings of the National Academy of Sciences of the United States of America·Radostin DanevWolfgang Baumeister
Apr 10, 2015·Nature·Candice E PaulsenDavid Julius
Apr 25, 2015·Cell·Yifan ChengThomas Walz
Aug 31, 2016·Methods in Enzymology·G McMullanR Henderson
Sep 16, 2016·Nature·Rafael Fernandez-Leiro, Sjors H W Scheres

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Citations

Jan 23, 2019·Protein Science : a Publication of the Protein Society·Risako TamuraTerukazu Nogi
May 8, 2019·Acta Crystallographica. Section D, Structural Biology·Evgenia A Markova, Giulia Zanetti
May 16, 2019·The Journal of Biological Chemistry·Guy Lippens, Benoît Gigant
Jul 12, 2019·Proceedings of the National Academy of Sciences of the United States of America·Claudio SchmidliThomas Braun
Nov 14, 2019·Experimental Biology and Medicine·Thomas B Thompson, Rhett A Kovall
Jan 8, 2020·Acta Crystallographica. Section D, Structural Biology·Lingxiao ZengQuan Hao
Nov 5, 2019·The Journal of Chemical Physics·James R McBride, Sandra J Rosenthal
Dec 31, 2019·ELife·Nicole PoweleitOren S Rosenberg
Mar 21, 2020·Biochemical Society Transactions·Xavier MartinezMarc Baaden
Apr 2, 2020·Protein Science : a Publication of the Protein Society·Mark K van GoorJenny van der Wijst
Jun 23, 2020·Annual Review of Biochemistry·Christoph Thomas, Robert Tampé
Jul 16, 2020·Nature Chemical Biology·Ricky C Cheng, Merritt Maduke
Mar 6, 2019·Nature Communications·Mark A HerzikGabriel C Lander
Dec 20, 2019·ELife·Qingtong ZhouSuwen Zhao
Dec 20, 2019·Protein Science : a Publication of the Protein Society·Xu Benjin, Liu Ling
Jan 28, 2020·British Journal of Pharmacology·Robert Eckenstaler, Ralf A Benndorf
Apr 29, 2020·Scientific Reports·Thales F D FernandesLuca Costa
May 21, 2020·Viral Immunology·Dongdong Cao, Bo Liang
Jul 2, 2020·Pflügers Archiv : European journal of physiology·Emilio Carbone, Yasuo Mori
May 31, 2019·Advanced Materials·Mario M ModenaStefan Wuttke
Dec 24, 2019·National Science Review·Manfred Auer
Mar 22, 2020·Proceedings of the National Academy of Sciences of the United States of America·R Andrew Byrd
Dec 5, 2019·International Journal of Molecular Sciences·Udo Heinemann, Anja Schuetz
Mar 21, 2019·Annual Review of Biomedical Engineering·Jana OgnjenovićSriram Subramaniam
May 31, 2019·Genome Biology·Fábio C P NavarroMark Gerstein
Mar 5, 2019·F1000Research·James P Allen
Apr 8, 2020·Acta Crystallographica. Section D, Structural Biology·David P KleblStephen P Muench
Aug 29, 2019·Proceedings of the National Academy of Sciences of the United States of America·Zhou YinRichard H Ebright
Mar 20, 2020·Physiological Reviews·Marianela G DalghiGerard Apodaca
Jul 31, 2019·Nature Methods·Sai Raghavendra Maddhuri Venkata SubramaniyaDaisuke Kihara
May 2, 2020·Acta Crystallographica. Section D, Structural Biology·John H Beale
Oct 16, 2018·The Journal of Clinical Investigation·Stanley Perlman, Tom Gallagher
Sep 26, 2020·Viruses·Nicholas C Wu, Ian A Wilson
Oct 10, 2020·Science·Micah Rapp, Bridget Carragher
Oct 21, 2020·Nature Biotechnology·Tural AkselShawn M Douglas
Aug 28, 2020·Journal of Virology·Bo Liang
Jan 14, 2021·Acta Crystallographica. Section F, Structural Biology Communications·Ryuichi KatoToshiya Senda
Oct 15, 2020·Current Opinion in Structural Biology·Bentley WingertIvet Bahar

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