Single-particle tracking photoactivated localization microscopy for mapping single-molecule dynamics.

Methods in Enzymology
Suliana ManleyJennifer Lippincott-Schwartz

Abstract

Recent developments in single-molecule localization techniques using photoactivatable fluorescent proteins have allowed the probing of single-molecule motion in a living cell with high specificity, millisecond time resolution, and nanometer spatial resolution. Analyzing the dynamics of individual molecules at high densities in this manner promises to provide new insights into the mechanisms of many biological processes, including protein heterogeneity in the plasma membrane, the dynamics of cytoskeletal flow, and clustering of receptor complexes in response to signaling cues. Here we describe the method of single-molecule tracking photoactivated localization microscopy (sptPALM) and discuss how its use can contribute to a quantitative understanding of fundamental cellular processes.

Citations

May 9, 2012·Chemical Reviews·Fedor V Subach, Vladislav V Verkhusha
Feb 14, 2014·Histochemistry and Cell Biology·Virgile Adam
Jul 7, 2011·International Journal of Molecular Sciences·Sheng-Wen Chiu, Mark C Leake
Dec 18, 2015·Journal of the American Chemical Society·Romain BerardozziDominique Bourgeois
May 27, 2015·The Journal of Cell Biology·Carlas S SmithDavid Grunwald
Jan 11, 2017·Chemical Reviews·Toru KondoGabriela S Schlau-Cohen
Jun 13, 2012·Nature Methods·Raghuveer Parthasarathy
Jan 19, 2020·Applied and Environmental Microbiology·Asmaa A SadoonYong Wang
Sep 24, 2011·Nature Reviews. Molecular Cell Biology·Atsushi Miyawaki
May 31, 2012·Chemistry : a European Journal·Janet CusidoFrançisco M Raymo
Jun 20, 2013·Physical Chemistry Chemical Physics : PCCP·Françisco M Raymo
Oct 31, 2012·Chemistry : a European Journal·Erhan DenizSalvatore Sortino
Oct 24, 2020·Cellular and Molecular Life Sciences : CMLS·Marc Hernaiz-LlorensEduardo Soriano
Mar 16, 2021·The Journal of General Physiology·Kazuki ObashiShigeo Okabe
Apr 13, 2021·Frontiers in Bioengineering and Biotechnology·Maria Arista-RomeroLorenzo Albertazzi
Jul 3, 2021·International Journal of Molecular Sciences·Peter HobothPavel Hozák
Dec 25, 2010·Journal of the American Chemical Society·Ibrahim YildizFrançisco M Raymo
Sep 22, 2012·The Journal of Physical Chemistry. a·Subramani SwaminathanFrançisco M Raymo
Jan 6, 2012·Journal of the American Chemical Society·Stefania ImpellizzeriFrançisco M Raymo

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