PMID: 9533417Apr 9, 1998Paper

Single pericytes and pericytes in suspension are stimulated in a similar way by low-density lipoprotein

Journal of Hypertension
S SkinnerW Vetter

Abstract

Pericytes are regarded as the microvascular counterpart of smooth muscle cells and implicated in the regulation of blood pressure at the microvascular level. Ca2+ plays an important role in biochemical processes involved in blood pressure regulation and can be activated by low-density lipoprotein (LDL) cholesterol. To determine whether stimulation either of single cells or of cells in suspension by LDL would produce any difference in the increase in cytosolic free calcium levels ([Ca2+]i). Single pericytes were loaded with 2 micromol/l of the Ca2+-sensitive dye Indo-1/AM. The Indo-1 fluorescence was recorded at 405 nm (Ca2+-bound) and 485 nm (Ca2+-free) after stimulation with LDL. Pericytes in suspension were loaded with 2 micromol/l of the Ca2+-sensitive dye FURA-2/AM. The FURA-2 fluorescence kinetics were recorded at 340-380 nm. Ratios of fluorescence at the two wavelengths were transformed to [Ca2+]i. Basal [Ca2+] levels appeared to be higher in single cells (148+/-13 nmol/l, n = 20) than they were in cells in suspension (128+/-8 nmol/l, n = 25; P= 0.0078). After stimulation with LDL the increase in [Ca2+]i in both systems was about 220% above baseline. A clear dose dependency was seen for both systems. Single pericytes and ...Continue Reading

References

May 12, 1975·Analytical Biochemistry·T G RedgraveC E West
Nov 1, 1992·The American Journal of Physiology·B HimpensR Casteels
Jan 1, 1991·Journal of Hypertension·P SchellingD Ganten
Dec 1, 1989·Journal of Hypertension. Supplement : Official Journal of the International Society of Hypertension·A SachinidisW Vetter
Apr 6, 1989·The New England Journal of Medicine·D SteinbergJ L Witztum
Jul 1, 1985·The Journal of Cell Biology·I M Herman, P A D'Amore
Dec 1, 1968·Journal of Ultrastructure Research·J A Rhodin
Dec 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P Holvoet, D Collen
Apr 1, 1993·The Journal of Clinical Investigation·K BoströmL L Demer

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