PMID: 9433459Jan 20, 1998Paper

Single point dilution method for the quantitative analysis of antibodies to the gag24 protein of HIV-1

Journal of Immunological Methods
D O PalenzuelaO Ganzó

Abstract

In the present work a concept proposed in 1992 by Dopotka and Giesendorf was applied to the quantitative analysis of antibodies to the p24 protein of HIV-1 in infected asymptomatic individuals and AIDS patients. Two approaches were analyzed, a linear model OD = b0 + b1.log(titer) and a nonlinear log(titer) = alpha.OD beta, similar to the Dopotka-Giesendorf's model. The above two proposed models adequately fit the dependence of the optical density values at a single point dilution, and titers achieved by the end point dilution method (EPDM). Nevertheless, the nonlinear model better fits the experimental data, according to residuals analysis. Classical EPDM was compared with the new single point dilution method (SPDM) using both models. The best correlation between titers calculated using both models and titers achieved by EPDM was obtained with the nonlinear model. The correlation coefficients for the nonlinear and linear models were r = 0.85 and r = 0.77, respectively. A new correction factor was introduced into the nonlinear model and this reduced the day-to-day variation of titer values. In general, SPDM saves time, reagents and is more precise and sensitive to changes in antibody levels, and therefore has a higher resolution...Continue Reading

References

Jan 1, 1992·Journal of Clinical Laboratory Analysis·H D Dopatka, B Giesendorf
Jun 24, 1992·Journal of Immunological Methods·A J Pesce, J G Michael
Jun 24, 1992·Journal of Immunological Methods·D M Kemeny
Jun 24, 1992·Journal of Immunological Methods·J E ButlerL A Cantarero
Dec 1, 1988·Journal of Virological Methods·J Husson-van Vliet, P Roussel
May 27, 1983·Journal of Immunological Methods·S M KramerN E Cremer
Jan 1, 1980·Journal of Immunoassay·D de Savigny, A Voller

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Citations

Oct 6, 1998·Journal of Immunological Methods·S J Merrill

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