Single potential electrophoresis microchip with reduced bias using pressure pulse injection

Electrophoresis
Frédéric Lacharme, Martin A M Gijs

Abstract

We propose two variants of a new injection technique for use in electrophoresis microchips, called "front gate pressure injection" and "back gate pressure injection", that both enable a controlled and reproducible sample introduction with reduced bias compared to electrokinetic gated injection. A continuous flow of a test solution of fluorescein/rhodamine B in 20 mM Tris/boric acid buffer (pH 8.6) sample test solution is electrokinetically driven near to the entrance of the separation channel, using a single voltage (3 kV) that is constant in time. A sample plug is injected in the separation channel by a pressure pulse of the order of 0.1 s. The latter is generated using the mechanical deflection of a PDMS membrane that is loosely placed on a dedicated chip reservoir. The analysis of the peak area ratio of the separated compounds demonstrates a nearly constant sample composition when using pressure-based injection. A small remaining injection bias for the shortest membrane deflection times can be attributed to a dilution effect of the charged compound due to the presence of an electrical field transverse to the sample flow boundary in the channel junction.

References

Apr 1, 1995·Electrophoresis·S C Jacobson, J M Ramsey
Oct 15, 1995·Analytical Chemistry·A T Woolley, R A Mathies
Feb 1, 1997·Analytical Chemistry·Q XueB L Karger
Nov 22, 1997·Journal of Chromatography. a·C L ColyerD J Harrison
Jul 25, 2000·Analytical Chemistry·N J MunroJ P Landers
Feb 24, 2001·Analytical Chemistry·E T LagallyR A Mathies
Apr 6, 2001·Electrophoresis·J P AlarieJ M Ramsey
Jun 14, 2001·Analytical Chemistry·C X Zhang, A Manz
Jul 2, 2002·Analytical Chemistry·Darwin R ReyesAndreas Manz
Jul 2, 2002·Analytical Chemistry·Pierre-Alain AurouxAndreas Manz
Aug 30, 2002·Analytical Chemistry·Ulli BackofenCraig E Lunte
Sep 28, 2002·Analytical Chemistry·Benjamin E SlentzFred Regnier
Oct 17, 2002·Analytical Chemistry·L M FuH H Liu
Apr 11, 2003·Journal of Colloid and Interface Science·David SintonDongqing Li
Oct 7, 2003·Journal of Colloid and Interface Science·David SintonDongqing Li
Apr 22, 2004·Lab on a Chip·Xiaoxia BaiHubert H Girault
Jun 15, 2004·Analytical Chemistry·Torsten VilknerAndreas Manz
Jul 23, 2004·Lab on a Chip·C FüttererJ-L Viovy
Feb 11, 2005·Journal of Chromatography. a·Seung Il ChoYong-Kweon Kim
Jun 1, 2005·Analytical Chemistry·James M KarlinseyJames P Landers
Oct 31, 2008·Talanta·Christopher T CulbertsonJ Michael Ramsey

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Citations

Jan 22, 2008·Journal of Chromatography. a·Dapeng WuBingcheng Lin
Apr 4, 2008·Electrophoresis·Lung-Ming FuChe-Hsin Lin
Nov 14, 2016·Talanta·Ellen F M GabrielWendell K T Coltro
May 24, 2008·Analytical Chemistry·Jonathan WestAndreas Manz

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