Single processing center models for human Dicer and bacterial RNase III

Cell
Haidi ZhangWitold Filipowicz

Abstract

Dicer is a multidomain ribonuclease that processes double-stranded RNAs (dsRNAs) to 21 nt small interfering RNAs (siRNAs) during RNA interference, and excises microRNAs from precursor hairpins. Dicer contains two domains related to the bacterial dsRNA-specific endonuclease, RNase III, which is known to function as a homodimer. Based on an X-ray structure of the Aquifex aeolicus RNase III, models of the enzyme interaction with dsRNA, and its cleavage at two composite catalytic centers, have been proposed. We have generated mutations in human Dicer and Escherichia coli RNase III residues implicated in the catalysis, and studied their effect on RNA processing. Our results indicate that both enzymes have only one processing center, containing two RNA cleavage sites and generating products with 2 nt 3' overhangs. Based on these and other data, we propose that Dicer functions through intramolecular dimerization of its two RNase III domains, assisted by the flanking RNA binding domains, PAZ and dsRBD.

References

Feb 7, 2001·Genes & Development·S M ElbashirT Tuschl
Nov 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·E BillyW Filipowicz
Feb 21, 2002·Molecular Biology of the Cell·Henrik MartensWolfgang Nellen
Jul 12, 2002·Nature·Gregory J Hannon
Oct 9, 2002·Genes & Development·Akira IshizukaHaruhiko Siomi
Nov 2, 2002·The EMBO Journal·Patrick ProvostOlof Rådmark
Nov 2, 2002·The EMBO Journal·Haidi ZhangWitold Filipowicz
Nov 6, 2002·Trends in Plant Science·Stephen E SchauerAnimesh Ray
Jul 19, 2003·Science·James C Carrington, Victor Ambros
Sep 26, 2003·Nature·Yoontae LeeV Narry Kim
Oct 7, 2003·Nature Genetics·Erno WienholdsRonald H A Plasterk
Oct 7, 2003·Nature Genetics·Emily BernsteinGregory J Hannon
Nov 7, 2003·Nucleic Acids Research·Eugenia BasyukEdouard Bertrand
Nov 15, 2003·Nature·Andreas LingelMichael Sattler
Nov 15, 2003·Nature·Kelley S YanMing-Ming Zhou
Mar 24, 2004·Cell·Yukihide TomariPhillip D Zamore

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Citations

Jan 6, 2011·JAMA : the Journal of the American Medical Association·Thomas Rio FrioMarc Tischkowitz
May 25, 2011·Cellular and Molecular Life Sciences : CMLS·Julia Starega-RoslanWlodzimierz J Krzyzosiak
Nov 26, 2005·Current Genetics·Michael Schroda
May 13, 2006·Current Genetics·Heriberto Cerutti, J Armando Casas-Mollano
Feb 18, 2009·Functional & Integrative Genomics·Qingpo LiuZhujun Zhu
Dec 13, 2007·Pharmaceutical Research·Yong ChenRam I Mahato
May 30, 2006·Virus Genes·Yehuda Stram, Larisa Kuzntzova
Jul 27, 2007·Journal of Biomedical Science·Annalisa PalmieriFrancesco Carinci
Jan 17, 2013·Applied Biochemistry and Biotechnology·Rajan Katoch, Neelam Thakur
Apr 1, 2008·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Preeti Singh TeotiaNeeti Sanan Mishra
Jun 1, 2005·Current Opinion in Structural Biology·Witold FilipowiczRamesh S Pillai
Jun 14, 2005·Trends in Biochemical Sciences·David Baulcombe
Aug 19, 2010·Expert Reviews in Molecular Medicine·Connor PhalonJohn Nemunaitis
May 15, 2012·Journal of Proteome Research·Alexandra C Schrimpe-RutledgeThomas O Metz
Aug 6, 2009·Acta Pharmacologica Sinica·Carlos Fabián Flores-JassoLuis Vaca
Nov 9, 2004·Nature·Ahmet M DenliGregory J Hannon
Jun 24, 2005·Nature·Thimmaiah P ChendrimadaRamin Shiekhattar
Jun 23, 2012·Nature·Sofia FranciaFabrizio d'Adda di Fagagna
Dec 28, 2004·Nature Biotechnology·Despina SiolasMichele A Cleary
Aug 9, 2011·Nature Reviews. Cancer·Marieke van KouwenhoveReuven Agami
Jan 18, 2005·Nature Reviews. Molecular Cell Biology·Erik J Sontheimer
Apr 27, 2005·Nature Reviews. Molecular Cell Biology·V Narry Kim
Dec 22, 2006·Nature Reviews. Molecular Cell Biology·Tariq M Rana
Jan 24, 2009·Nature Reviews. Molecular Cell Biology·V Narry KimMikiko C Siomi
Jun 27, 2013·Nature Reviews. Molecular Cell Biology·Stefan L Ameres, Phillip D Zamore
Jan 20, 2009·Nature Structural & Molecular Biology·Weiyang ShiBenjamin Haley
Aug 12, 2009·Nature Structural & Molecular Biology·Patricia LandryPatrick Provost
Jun 21, 2011·Nature Structural & Molecular Biology·Frédérique RauNicolas Charlet-Berguerand
Mar 20, 2012·Nature Structural & Molecular Biology·Pick-Wei LauIan J MacRae
Sep 18, 2007·Nature Structural & Molecular Biology·Ian J MacRaeJennifer A Doudna
Sep 25, 2007·Nature Structural & Molecular Biology·Florian A SteinerTitia Sijen
Feb 21, 2007·Cell Research·Zain ParooXiaodong Wang
Jul 11, 2007·Immunology and Cell Biology·Michael P GantierBryan R G Williams
Aug 30, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Masayuki SanoJohn J Rossi
Sep 5, 2006·Molecular BioSystems·Paul Workman
Dec 23, 2011·The New England Journal of Medicine·Alireza Heravi-MoussaviDavid G Huntsman
Jan 8, 2008·Proceedings of the National Academy of Sciences of the United States of America·Ian J MacRaeJennifer A Doudna
Feb 13, 2008·Proceedings of the National Academy of Sciences of the United States of America·Zhihua DuThomas L James
Oct 10, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kristin L PatrickElisabetta Ullu

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