Single pump column switching technique employing a flow gradient and wavelength programmed fluorescence for simultaneous monitoring of serotonin, fluoxetine and norfluoxetine in rat brain microdialysate

Journal of Pharmaceutical and Biomedical Analysis
A RamaiyaH T Karnes

Abstract

A single pump column switching technique with multidimensional chromatography, flow gradient and wavelength programmed fluorescence detection was developed for simultaneous quantitation of serotonin, fluoxetine and norfluoxetine in rat brain microdialysate. The column switching was configured such that position I of the switching valve employed column I (50 mm length) and column II (250 mm length) in series. This configuration resulted in optimal resolution of serotonin from interfering neurochemicals from rat brain. After elution of serotonin at 13.2 min the valve was switched to position II in which the flow of the mobile phase was directed through column I only. Flow gradient programming was then used to ramp the flow rate from 0.1 to 0.4 ml min-1 which resulted in optimal elution of fluoxetine and norfluoxetine. Strategic optimization of the single mobile phase enabled use of a single pump and detector making the analytical system simple and cost effective. Wavelength programmed fluorescence enabled sensitive detection of the analytes despite the difference in their fluorescence spectrum. The limit of detection for serotonin, norfluoxetine and fluoxetine were 10, 612 and 523 fmol, respectively. Rat brain microdialysate samp...Continue Reading

References

Jan 1, 1991·Journal of Pharmaceutical and Biomedical Analysis·H T Karnes, C March
Oct 1, 1990·Oral Surgery, Oral Medicine, and Oral Pathology·W IchikawaH M Rosenberg
Jan 10, 1984·The Journal of Comparative Neurology·J Szabo, W M Cowan

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Citations

Oct 9, 2002·Journal of Pharmaceutical and Biomedical Analysis·Mohamed A El-dawyFawzy A El-Barbary
Feb 23, 2005·Chemical Communications : Chem Comm·Brett PaullPavel N Nesterenko

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