Single-step immobilization of cell adhesive peptides on a variety of biomaterial substrates via tyrosine oxidation with copper catalyst and hydrogen peroxide

Bioconjugate Chemistry
Sachiro Kakinoki, Tetsuji Yamaoka

Abstract

Immobilization of biologically active peptides which were isolated from extracellular matrix proteins is a powerful strategy for the design and functionalization of biomaterial substrates. However, the method of peptide immobilization was restricted, that is, peptide is often immobilized through the reactive groups inherent in substrates with multistep reactions. Here, we report a single-step immobilization of fibronectin-derived cell adhesive peptide (Arg-Glu-Asp-Val; REDV) onto polymer materials by use of tyrosine oxidation with copper catalyst and hydrogen peroxide. REDV peptide was successfully immobilized on tissue culture polystyrene, poly(ethylene terephthalate), poly(vinyl chloride), expanded-poly(tetrafluoroethylene), and poly(l-lactic acid), resulting in enhanced adhesion of human umbilical vein endothelial cells. This method is a single-step reaction under very mild conditions and is available for the biological functionalization of various medical devices.

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Citations

Sep 17, 2016·Biological Chemistry·Mareen Pagel, Annette G Beck-Sickinger
Oct 5, 2017·Journal of Biomedical Materials Research. Part a·Sachiro KakinokiTetsuji Yamaoka
Dec 30, 2017·Bone Research·Chengde GaoCijun Shuai
Feb 7, 2020·Journal of Polymer Science. Part B, Polymer Physics·Bastiaan D IppelPatricia Y W Dankers
Apr 28, 2020·Journal of Biomaterials Science. Polymer Edition·Yuichi OhyaTetsuji Yamaoka
Jul 25, 2018·Chemical Reviews·Christopher D SpicerMolly M Stevens
Sep 19, 2019·Bioconjugate Chemistry·Franziska ClauderAnnette G Beck-Sickinger

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