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Single-step isolation and resolution of pancreatic carboxypeptidases A and B
Biochemistry
Oct 2, 1979
T J BazzoneBert L Vallee
PMID: 486428
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Abstract
Carboxypeptidases A and B have been isolated individually from aqueous extracts of mammalian pancreatic acetone powders by affinity chromatography on [N-(epsilon-aminocaproyl)-p-aminobenzyl]succinyl-Sepharose 4B (CABS-Sepharose). The affinity ligand was synthesized from DL-benzylsuccini...read more
Mentioned in this Paper
Enzymes, antithrombotic
Enzymes, peripheral vasodilators
Pancreatic Juice
Sepharose 4B
Ligands
Enzymes, hematological
Sepharose
Polyacrylamide Gel Electrophoresis
Carboxypeptidase A Activity
Chromatography, Affinity
Paper Details
References
- References16
- Citations17
Selective enzyme purification by affinity chromatography
Proceedings of the National Academy of Sciences of the United States of AmericaOctober 1, 1968Pedro CuatrecasasC B Anfinsen12191Adsorbents for affinity chromatography. Use of N-hydroxysuccinimide esters of agarose
BiochemistryJune 6, 1972Pedro Cuatrecasas, I K Parikh60FUNCTIONAL TYROSYL RESIDUES IN THE ACTIVE CENTER OF BOVINE PANCREATIC CARBOXYPEPTIDASE A
BiochemistryJanuary 1, 1963Robert T SimpsonBert L ValleeBinding of the by-product analog benzylsuccinic acid by carboxypeptidase A
BiochemistryMay 22, 1973Larry D Byers, Richard V WolfendenPROCARBOXYPEPTIDASE A-S6. FURTHER STUDIES OF ITS ISOLATION AND PROPERTIES
BiochemistryJanuary 1, 1963Masayuki YamasakiHartmud NeurathQuantitative affinity chromatography. Determination of binding constants by elution with competitive inhibitors
Proceedings of the National Academy of Sciences of the United States of AmericaJune 1, 1974Benn M Dunn, Irwin M Chaiken20Kinetics of carboxypeptidase A. II. Inhibitors of the hydrolysis of oligopeptides
BiochemistryFebruary 3, 1970Douglas S Auld, Bert L ValleePurification and crystallization of human carboxypeptidase A
BiochemistryJune 15, 1976Leif PetersonBert L ValleeIntrinsic enzymatic activity of bovine procarboxypeptidase A S5
BiochemistryAugust 13, 1974J R Uren, Hartmud NeurathMechanism of activation of bovine procarboxypeptidase A S 5 . Alterations in primary and quaternary structure
BiochemistryNovember 21, 1972J R Uren, Hartmud Neurath
12
- References16
- Citations17
Cryokinetic studies of the intermediates in the mechanism of carboxypeptidase A
BiochemistryApril 12, 1983A GaldesBert L ValleeCarboxypeptidase A: mechanism of zinc inhibition
BiochemistryDecember 12, 1989Kim S Larsen, Douglas S Auld
Interaction of cobalt ions with carboxypeptidase A
Journal of Inorganic BiochemistryJanuary 1, 1994José María MoratalR Domingo13C NMR studies of D- and L-phenylalanine binding to cobalt(II) carboxypeptidase A
Journal of Inorganic BiochemistryJanuary 1, 1988Claudio LuchinatDouglas S Auld1H NMR spectroscopic characterization of binary and ternary complexes of cobalt(II) carboxypeptidase A with inhibitors
BiochemistryNovember 1, 1988Ivano BertiniJames F RiordanCatalytic and conformational changes induced by limited subtilisin cleavage of bovine carboxypeptidase A
BiochemistryAugust 7, 1990Beka SolomonJames F Riordan
Primary structure of carboxypeptidase T: delineation of functionally relevant features in Zn-carboxypeptidase family
Journal of Protein ChemistryOctober 1, 1992Andrei OstermanViktor Stepanov301
A unique activity assay for carboxypeptidase A in human serum
Analytical BiochemistrySeptember 15, 1982Leif PetersonJ L Bethune
Purification and characterization of a proteinase from Euphausia superba Dana (Antarctic krill)
Acta biochimica PolonicaJanuary 1, 1986M TurkiewiczMałgorzata ZielińskaAffinity chromatographic sorting of carboxypeptidase A and its chemically modified derivatives
Analytical BiochemistrySeptember 15, 1980L B CueniBert L Vallee
12
Single-step isolation and resolution of pancreatic carboxypeptidases A and B
Abstract
Carboxypeptidases A and B have been isolated individually from aqueous extracts of mammalian pancreatic acetone powders by affinity chromatography on [N-(epsilon-aminocaproyl)-p-aminobenzyl]succinyl-Sepharose 4B (CABS-Sepharose). The affinity ligand was synthesized from DL-benzylsuccini...read more
Mentioned in this Paper
Enzymes, antithrombotic
Enzymes, peripheral vasodilators
Pancreatic Juice
Sepharose 4B
Ligands
Paper Details
References
- References16
- Citations17
Selective enzyme purification by affinity chromatography
Proceedings of the National Academy of Sciences of the United States of AmericaOctober 1, 1968Pedro CuatrecasasC B Anfinsen12191Adsorbents for affinity chromatography. Use of N-hydroxysuccinimide esters of agarose
BiochemistryJune 6, 1972Pedro Cuatrecasas, I K Parikh60FUNCTIONAL TYROSYL RESIDUES IN THE ACTIVE CENTER OF BOVINE PANCREATIC CARBOXYPEPTIDASE A
BiochemistryJanuary 1, 1963Robert T SimpsonBert L ValleeBinding of the by-product analog benzylsuccinic acid by carboxypeptidase A
BiochemistryMay 22, 1973Larry D Byers, Richard V WolfendenPROCARBOXYPEPTIDASE A-S6. FURTHER STUDIES OF ITS ISOLATION AND PROPERTIES
BiochemistryJanuary 1, 1963Masayuki YamasakiHartmud NeurathQuantitative affinity chromatography. Determination of binding constants by elution with competitive inhibitors
Proceedings of the National Academy of Sciences of the United States of AmericaJune 1, 1974Benn M Dunn, Irwin M Chaiken20Kinetics of carboxypeptidase A. II. Inhibitors of the hydrolysis of oligopeptides
BiochemistryFebruary 3, 1970Douglas S Auld, Bert L ValleePurification and crystallization of human carboxypeptidase A
BiochemistryJune 15, 1976Leif PetersonBert L ValleeIntrinsic enzymatic activity of bovine procarboxypeptidase A S5
BiochemistryAugust 13, 1974J R Uren, Hartmud NeurathMechanism of activation of bovine procarboxypeptidase A S 5 . Alterations in primary and quaternary structure
BiochemistryNovember 21, 1972J R Uren, Hartmud Neurath
12
- References16
- Citations17
Cryokinetic studies of the intermediates in the mechanism of carboxypeptidase A
BiochemistryApril 12, 1983A GaldesBert L ValleeCarboxypeptidase A: mechanism of zinc inhibition
BiochemistryDecember 12, 1989Kim S Larsen, Douglas S AuldInteraction of cobalt ions with carboxypeptidase A
Journal of Inorganic BiochemistryJanuary 1, 1994José María MoratalR Domingo13C NMR studies of D- and L-phenylalanine binding to cobalt(II) carboxypeptidase A
Journal of Inorganic BiochemistryJanuary 1, 1988Claudio LuchinatDouglas S Auld1H NMR spectroscopic characterization of binary and ternary complexes of cobalt(II) carboxypeptidase A with inhibitors
BiochemistryNovember 1, 1988Ivano BertiniJames F RiordanCatalytic and conformational changes induced by limited subtilisin cleavage of bovine carboxypeptidase A
BiochemistryAugust 7, 1990Beka SolomonJames F RiordanPrimary structure of carboxypeptidase T: delineation of functionally relevant features in Zn-carboxypeptidase family
Journal of Protein ChemistryOctober 1, 1992Andrei OstermanViktor Stepanov301A unique activity assay for carboxypeptidase A in human serum
Analytical BiochemistrySeptember 15, 1982Leif PetersonJ L BethunePurification and characterization of a proteinase from Euphausia superba Dana (Antarctic krill)
Acta biochimica PolonicaJanuary 1, 1986M TurkiewiczMałgorzata ZielińskaAffinity chromatographic sorting of carboxypeptidase A and its chemically modified derivatives
Analytical BiochemistrySeptember 15, 1980L B CueniBert L Vallee
12
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