Single-step isolation and resolution of pancreatic carboxypeptidases A and B
Biochemistry
Oct 2, 1979
T J BazzoneBert L Vallee
PMID: 486428
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Abstract
Carboxypeptidases A and B have been isolated individually from aqueous extracts of mammalian pancreatic acetone powders by affinity chromatography on [N-(epsilon-aminocaproyl)-p-aminobenzyl]succinyl-Sepharose 4B (CABS-Sepharose). The affinity ligand was synthesized from DL-benzylsuccini...read more
Mentioned in this Paper
Enzymes, antithrombotic
Enzymes, peripheral vasodilators
Pancreatic Juice
Sepharose 4B
Ligands
Enzymes, hematological
Sepharose
Polyacrylamide Gel Electrophoresis
Carboxypeptidase A Activity
Chromatography, Affinity
Paper Details
References
- References16
- Citations17
Kinetics of carboxypeptidase A. II. Inhibitors of the hydrolysis of oligopeptides
BiochemistryFebruary 3, 1970Douglas S Auld, Bert L ValleeBovine procarboxypeptidase A: kinetics of peptide and ester hydrolysis
BiochemistryFebruary 24, 1976T J Bazzone, Bert L ValleeAdsorbents for affinity chromatography. Use of N-hydroxysuccinimide esters of agarose
BiochemistryJune 6, 1972Pedro Cuatrecasas, I K Parikh61Intrinsic enzymatic activity of bovine procarboxypeptidase A S5
BiochemistryAugust 13, 1974J R Uren, Hartmud NeurathMechanism of activation of bovine procarboxypeptidase A S 5 . Alterations in primary and quaternary structure
BiochemistryNovember 21, 1972J R Uren, Hartmud Neurath
Quantitative affinity chromatography. Determination of binding constants by elution with competitive inhibitors
Proceedings of the National Academy of Sciences of the United States of AmericaJune 1, 1974Benn M Dunn, Irwin M Chaiken20FUNCTIONAL TYROSYL RESIDUES IN THE ACTIVE CENTER OF BOVINE PANCREATIC CARBOXYPEPTIDASE A
BiochemistryJanuary 1, 1963Robert T SimpsonBert L Vallee
Affinity chromatography of proteolytic enzymes
Biochimica Et Biophysica ActaApril 27, 1971J R UrenIsolation and characterization of carboxypeptidases A and B from activated pancreatic juice
BiochemistryDecember 7, 1971Gerald R ReeckH NeurathBinding of the by-product analog benzylsuccinic acid by carboxypeptidase A
BiochemistryMay 22, 1973Larry D Byers, Richard V Wolfenden
12
- References16
- Citations17
Purification and characterization of a proteinase from Euphausia superba Dana (Antarctic krill)
Acta biochimica PolonicaJanuary 1, 1986M TurkiewiczMałgorzata Zielińska
13C NMR studies of carboxylate inhibitor binding to cobalt(II) carboxypeptidase A
Journal of Inorganic BiochemistryJanuary 1, 1988Ivano BertiniDouglas S AuldAffinity chromatographic purification of angiotensin converting enzyme
BiochemistryFebruary 28, 1984Michael W PantolianoJames F RiordanCatalytic and conformational changes induced by limited subtilisin cleavage of bovine carboxypeptidase A
BiochemistryAugust 7, 1990Beka SolomonJames F RiordanCarboxypeptidase A: mechanism of zinc inhibition
BiochemistryDecember 12, 1989Kim S Larsen, Douglas S Auld1H NMR spectroscopic characterization of binary and ternary complexes of cobalt(II) carboxypeptidase A with inhibitors
BiochemistryNovember 1, 1988Ivano BertiniJames F Riordan
Purification of carboxypeptidase B by metal chelation affinity chromatography
Journal of Biochemical and Biophysical MethodsMarch 1, 1992M Márquez-Méndez
Affinity chromatographic sorting of carboxypeptidase A and its chemically modified derivatives
Analytical BiochemistrySeptember 15, 1980L B CueniBert L ValleeA unique activity assay for carboxypeptidase A in human serum
Analytical BiochemistrySeptember 15, 1982Leif PetersonJ L Bethune13C NMR studies of D- and L-phenylalanine binding to cobalt(II) carboxypeptidase A
Journal of Inorganic BiochemistryJanuary 1, 1988Claudio LuchinatDouglas S Auld
12
Related Papers
Kinetics of carboxypeptidase A. II. Inhibitors of the hydrolysis of oligopeptides
BiochemistryFebruary 3, 1970
Douglas S Auld, Bert L Vallee
PROCEDURES FOR THE ISOLATION OF CRYSTALLINE BOVINE PANCREATIC CARBOXYPEPTIDASE A. II. ISOLATION OF CARBOXYPEPTIDASE A-ALPHA FROM PROCARBOXYPEPTIDASE A
BiochemistryJanuary 1, 1964
David G CoxHartmud Neurath
Single-step isolation and resolution of pancreatic carboxypeptidases A and B
Abstract
Carboxypeptidases A and B have been isolated individually from aqueous extracts of mammalian pancreatic acetone powders by affinity chromatography on [N-(epsilon-aminocaproyl)-p-aminobenzyl]succinyl-Sepharose 4B (CABS-Sepharose). The affinity ligand was synthesized from DL-benzylsuccini...read more
Mentioned in this Paper
Enzymes, antithrombotic
Enzymes, peripheral vasodilators
Pancreatic Juice
Sepharose 4B
Ligands
Related Papers
Paper Details
References
- References16
- Citations17
Kinetics of carboxypeptidase A. II. Inhibitors of the hydrolysis of oligopeptides
BiochemistryFebruary 3, 1970Douglas S Auld, Bert L ValleeBovine procarboxypeptidase A: kinetics of peptide and ester hydrolysis
BiochemistryFebruary 24, 1976T J Bazzone, Bert L ValleeAdsorbents for affinity chromatography. Use of N-hydroxysuccinimide esters of agarose
BiochemistryJune 6, 1972Pedro Cuatrecasas, I K Parikh61Intrinsic enzymatic activity of bovine procarboxypeptidase A S5
BiochemistryAugust 13, 1974J R Uren, Hartmud NeurathMechanism of activation of bovine procarboxypeptidase A S 5 . Alterations in primary and quaternary structure
BiochemistryNovember 21, 1972J R Uren, Hartmud NeurathQuantitative affinity chromatography. Determination of binding constants by elution with competitive inhibitors
Proceedings of the National Academy of Sciences of the United States of AmericaJune 1, 1974Benn M Dunn, Irwin M Chaiken20FUNCTIONAL TYROSYL RESIDUES IN THE ACTIVE CENTER OF BOVINE PANCREATIC CARBOXYPEPTIDASE A
BiochemistryJanuary 1, 1963Robert T SimpsonBert L ValleeAffinity chromatography of proteolytic enzymes
Biochimica Et Biophysica ActaApril 27, 1971J R UrenIsolation and characterization of carboxypeptidases A and B from activated pancreatic juice
BiochemistryDecember 7, 1971Gerald R ReeckH NeurathBinding of the by-product analog benzylsuccinic acid by carboxypeptidase A
BiochemistryMay 22, 1973Larry D Byers, Richard V Wolfenden
12
- References16
- Citations17
Purification and characterization of a proteinase from Euphausia superba Dana (Antarctic krill)
Acta biochimica PolonicaJanuary 1, 1986M TurkiewiczMałgorzata Zielińska13C NMR studies of carboxylate inhibitor binding to cobalt(II) carboxypeptidase A
Journal of Inorganic BiochemistryJanuary 1, 1988Ivano BertiniDouglas S AuldAffinity chromatographic purification of angiotensin converting enzyme
BiochemistryFebruary 28, 1984Michael W PantolianoJames F RiordanCatalytic and conformational changes induced by limited subtilisin cleavage of bovine carboxypeptidase A
BiochemistryAugust 7, 1990Beka SolomonJames F RiordanCarboxypeptidase A: mechanism of zinc inhibition
BiochemistryDecember 12, 1989Kim S Larsen, Douglas S Auld1H NMR spectroscopic characterization of binary and ternary complexes of cobalt(II) carboxypeptidase A with inhibitors
BiochemistryNovember 1, 1988Ivano BertiniJames F RiordanPurification of carboxypeptidase B by metal chelation affinity chromatography
Journal of Biochemical and Biophysical MethodsMarch 1, 1992M Márquez-MéndezAffinity chromatographic sorting of carboxypeptidase A and its chemically modified derivatives
Analytical BiochemistrySeptember 15, 1980L B CueniBert L ValleeA unique activity assay for carboxypeptidase A in human serum
Analytical BiochemistrySeptember 15, 1982Leif PetersonJ L Bethune13C NMR studies of D- and L-phenylalanine binding to cobalt(II) carboxypeptidase A
Journal of Inorganic BiochemistryJanuary 1, 1988Claudio LuchinatDouglas S Auld
12
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