Single-step PCR for detection of Brucella spp. from blood and milk of infected animals.

Journal of Clinical Microbiology
D S Leal-KlevezasJ P Martínez-Soriano

Abstract

A versatile method for the extraction of Brucella DNA and PCR are presented as reliable tools for the detection of Brucella spp. from body fluids of infected animals. Two oligonucleotides homologous to regions of the gene encoding for an outer membrane protein (omp-2) were designed to detect the pathogen from milk and/or blood of infected goats, bovines, and human patients. The sensitivity of our test and its ability to detect the pathogen in samples from the field reveal a promising advance in the diagnosis of brucellosis in animals and humans.

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Citations

Mar 21, 2009·Journal of Applied Microbiology·R CapparelliD Iannelli
Nov 23, 2019·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Juliana P S MolRenato L Santos
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Brucellosis is a bacterial infection caused by members of the genus brucella and remains one of the world's major zoonotic diseases. Discover the latest research on Brucellosis here.

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