Single-step separation of bioactive flavonol glucosides from Osteomeles schwerinae by high-speed counter-current chromatography

Journal of Separation Science
Jun LeeJin Sook Kim

Abstract

Two bioactive flavonol glucosides, hyperoside and quercitrin, were successfully isolated in one step from the phytochemically unknown medicinal plant Osteomeles schwerinae by high-speed counter-current chromatography using a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (0.5:5.5:1.5:4.5 v/v). From 160 mg of crude extract, 2.1 mg of hyperoside (98.6% purity, 83.3% recovery) and 4.5 mg of quercitrin (99.2% purity, 81.7% recovery) were separated and then their chemical structures were identified by (1)H and (13)C NMR analysis. In addition, the isolates were subjected to an in vitro bioassay to evaluate their inhibitory activity on rat lens aldose reductase. Both quercitrin and hyperoside showed excellent inhibitory activities toward rat lens aldose reductase with the IC(50) values of 0.16 and 4.33 microM, respectively, as compared with positive control, 3,3-tetramethyleneglutaric acid (28.7 microM). So far, chemical constituents and biological activities of O. schwerinae have never been reported. This is the first report on the chemical constituents and biological activity of this plant using semi-preparative high-speed counter-current chromatography separation technique.

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Citations

Dec 7, 2013·BioMed Research International·Ji Hun PaekSoon Sung Lim
Mar 13, 2014·Journal of Chromatographic Science·Jia LiWeina Wang
Mar 24, 2016·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Young Sook KimJin Sook Kim
Jan 25, 2013·Nutrition Research and Practice·Hyo Jung KimJong-Sang Kim
Apr 24, 2020·Natural Product Research·Josiane A Monteiro de OliveiraDebora Cristina Baldoqui
Jun 7, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Bo-Jeong PyunJin Sook Kim
Jun 1, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Eunjin SohnJin Sook Kim

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