PMID: 6162480Jan 20, 1981Paper

Single-strand and double-strand deoxyribonucleic acid breaks produced by several bleomycin analogues

Biochemistry
C H HuangS T Crooke

Abstract

Production of single-strand breaks (ssb) and double-strand breaks (dsb) of PM2 phage DNA by several structurally related bleomycin (BLM) analogues was studied by gel electrophoresis. BLM A2 and BLM B2 produced a comparable extent of dsb. In various experiments, BLM A2 and BLM B2, at 22-41 ng/mL, degraded 50% of the form I DNA into 33-38% form II and 12-17% form III DNA. BLM B1' produced ssb and dsb at a ratio similar to that of BLM A2, but both at a rate less than half that of BLM A2. Phleomycin (PLM) D1 induced an equivalent amount of ssb but only one-eighth of dsb induced by BLM B2. The relatively lower extent dsb production for PLM D1 was observed either in borate buffer (pH 9.5) or in Tris-HCl buffer (pH 7.5) and in the presence or absence of exogenous Fe(II). Deamido-BLM A2 produced ssb to an extent approximately half that of BLM A2 and dsb to less than one-eighth that of BLM A2. The following conclusions were drawn. (1) BLM analogues produced ssb and dsb to different extents and ratios. (2) The ratio of dsb to ssb varied depending on the analogue, indicating a lack of a direct correlation between ssb and dsb. (3) The extent of ssb and dsb was affected by modifications on both the C- and N-terminal half-molecules of BLM: m...Continue Reading

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Citations

Jan 1, 1982·Journal of Cancer Research and Clinical Oncology·H MuliawanH Kappus
Jan 1, 1982·Journal of Cancer Research and Clinical Oncology·P K ChanH Busch
Jun 1, 1994·Cancer Metastasis Reviews·B A Teicher
Mar 1, 1983·Somatic Cell Genetics·T D StamatoL Mackenzie
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Feb 3, 2005·Nature Reviews. Cancer·Jingyang Chen, JoAnne Stubbe
Jun 5, 2013·Current Genetic Medicine Reports·Benjamin B CurrallCynthia C Morton
Mar 1, 1991·Mutation Research·L F Povirk, M J Austin
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Mar 26, 2016·Micron : the International Research and Review Journal for Microscopy·Brian M Preziosi, Timothy J Bowden
Feb 1, 1983·Chemico-biological Interactions·J R PellonR F Gomez
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Sep 24, 2016·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·La Ode Muhammad Muchdar DavisMasaaki Umeda
Aug 27, 2019·Plant Signaling & Behavior·Hidenori TanakaAkihiro Imai
Oct 13, 2017·Nature Communications·Mariano OppikoferAndrea G Cochran
Oct 21, 2014·Chemical Communications : Chem Comm·Megha Subhash DeshpandeShun Hirota
Aug 25, 2017·Radiation Oncology·Florian Wirsdörfer, Verena Jendrossek
May 8, 2018·International Journal of Molecular Sciences·Vincent MurrayLong H Chung
Aug 9, 2002·Journal of the American Chemical Society·Ying ZouSidney M Hecht

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