PMID: 9418243Jan 7, 1998Paper

Single strand conformation polymorphism analysis of PCR-tDNA fingerprinting to address the identification of Bacillus species

FEMS Microbiology Letters
S BorinC Sorlini

Abstract

The suitability of tDNA-PCR fingerprinting to identify species of the genus Bacillus was tested on 75 strains. Strains belonging to the same species or the same phylogenetic cluster were correctly grouped. Among B. stearothermophilus strains, different pattern types were found. This could be due to the unclear taxonomic situation of these strains, rather than to a failure of the tDNA-PCR. Single strand conformation polymorphism (SSCP) of the PCR products allowed species discrimination within the 'B. subtilis group', but not within the 'B. cereus group'. The tDNA-PCR, alone or coupled with SSCP analysis, is useful to address Bacillus species identification, particularly for those species which are not phylogenetically tightly clustered.

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Citations

Apr 13, 2007·Extremophiles : Life Under Extreme Conditions·Yasushi KageyamaKoki Horikoshi
Feb 18, 2004·Journal of Microbiological Methods·Myron T La DucKasthuri Venkateswaran
Aug 3, 2001·International Journal of Food Microbiology·G Giraffa, E Neviani
Dec 31, 2003·International Journal of Food Microbiology·Labia Irène Ivette OuobaPeter Lange Møller
Feb 24, 2001·Biosensors & Bioelectronics·S S IqbalJ P Chambers
Sep 21, 2010·Applied and Environmental Microbiology·Bessem ChouaiaClaudio Bandi
Mar 24, 2009·Applied and Environmental Microbiology·Ilias KounatidisKostas Bourtzis
Sep 6, 2003·Applied and Environmental Microbiology·Daniele DaffonchioSara Borin
Feb 8, 2006·Applied and Environmental Microbiology·Daniele DaffonchioSara Borin
Apr 3, 2001·Journal of Clinical Microbiology·A L McOristA R Bird
Feb 13, 2009·PloS One·Shalini PorwalVipin Chandra Kalia
Jul 30, 2005·FEMS Immunology and Medical Microbiology·Konstantinos KesanopoulosStamatina Levidiotou-Stefanou
Jan 10, 2017·Microbial Pathogenesis·Sana ShafiSuhaib A Bandh
Jan 16, 2008·Letters in Applied Microbiology·G CenciP Bosi
Sep 5, 2008·International Journal of Systematic and Evolutionary Microbiology·Clara UrzìPeter Schumann
Jul 25, 2007·Journal of Agricultural and Food Chemistry·Maria Jose ChapelaCarmen G Sotelo

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