Single-stranded DNA-binding proteins and neuron-restrictive silencer factor participate in cell-specific transcriptional control of the NMDAR1 gene.

The Journal of Biological Chemistry
G BaiJ W Kusiak

Abstract

Our previous studies revealed that a proximal region of the N-methyl-D-aspartate receptor 1 (NMDAR1) promoter is important for cell-type-specific expression. We have now explored the contributions of several regulatory elements to this specificity. Deletion of the neuron-restrictive silencer element partially relieved the suppression of promoter activity in C6 glioma and HeLa cells. An overlapping G(C/G)G/tandem Sp1-containing region crucial for both basal and nerve growth factor (NGF)-regulated promoter activity specifically bound nuclear proteins on its purine-rich sense strand. A faster migrating complex, single-stranded binding protein complex 1 (SBPC1), was highly enriched in HeLa cells, whereas a slower migrating complex, SBPC2, was enriched in PC12 cells. A high ratio of 2/1 complex correlated with a high level of promoter activity. NGF treatment of PC12 cells reduced SBPC1 but increased SBPC2. Competition experiments showed that the SBPC1 binding required a dG4 sequence and the SBPC2 needed a core of TG3A plus a 5'-flanking sequence. Single-stranded DNA encompassing TG3A and/or dG4 specifically suppressed cotransfected NMDAR1 promoter activity. UV cross-linking studies indicated that a 31.5-kDa protein mainly formed SBP...Continue Reading

References

Jul 2, 1992·Nature·T KutsuwadaM Arakawa
Sep 15, 1992·Biochemistry·T MinowaM M Mouradian
Mar 1, 1991·Journal of Neurochemistry·K Y GandelmanK L O'Malley
Nov 7, 1991·Nature·K MoriyoshiS Nakanishi
Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S L AckermanC Y Yeung
Jul 1, 1989·Trends in Neurosciences·S A Lipton, S B Kater
Jun 5, 1987·Science·T ManiatisJ A Fischer
Jul 1, 1995·Synapse·S R BilakD K Morest
Apr 21, 1995·The Journal of Biological Chemistry·E F MichelottiD Levens
May 5, 1995·The Journal of Biological Chemistry·M E Grossmann, D J Tindall
Feb 24, 1995·The Journal of Biological Chemistry·G H MacDonaldJ P Ting
Jan 1, 1994·Annual Review of Neuroscience·M Hollmann, S Heinemann
Feb 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·L LiP Greengard
Jan 7, 1993·Nature·T V Bliss, G L Collingridge
Oct 1, 1995·Current Opinion in Neurobiology·C J Schoenherr, D J Anderson
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·T Tomonaga, D Levens
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·C J SchoenherrD J Anderson
Feb 28, 1997·The Journal of Biological Chemistry·G Bai, J W Kusiak

❮ Previous
Next ❯

Citations

Mar 20, 2004·Pharmacology & Therapeutics·Janine L Steiger, Shelley J Russek
Nov 9, 2000·Molecular and Biochemical Parasitology·M R FantappieP T LoVerde
Oct 16, 1999·Brain Research. Molecular Brain Research·M CalbetL de Lecea
Jun 29, 1999·Cold Spring Harbor Symposia on Quantitative Biology·L B Rothman-DenesK Kazmierczak
Aug 19, 2010·Pharmacological Reviews·Stephen F TraynelisRay Dingledine
May 20, 1999·Annual Review of Pharmacology and Toxicology·S J MyersK Borges
Jun 13, 2014·PLoS Computational Biology·Shira RockowitzDeyou Zheng
Oct 12, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Mariusz MuchaIan C Wood
Jan 10, 2002·Biochemical and Biophysical Research Communications·Akiko TabuchiMasaaki Tsuda
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y NaruseN Mori
Feb 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Cecilia ConacoGail Mandel
Jun 24, 2008·Experimental Cell Research·Yinxiang YangXuetao Pei
Jul 31, 2003·Journal of Neurochemistry·Guang BaiPeter W Hoffman
Sep 29, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Priya VermaPoonam Singh
Nov 19, 2013·Biological Psychiatry·Subramaniam JayanthiJean Lud Cadet
Jan 14, 2004·The Journal of Biological Chemistry·Andrés CouveStephen J Moss
Mar 14, 2017·Frontiers in Cell and Developmental Biology·David Martin, Anne Grapin-Botton

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.