Single-stranded DNA oligomers stimulate error-prone alternative repair of DNA double-strand breaks through hijacking Ku protein

Nucleic Acids Research
Ying YuanPatrick Calsou

Abstract

In humans, DNA double-strand breaks (DSBs) are repaired by two mutually-exclusive mechanisms, homologous recombination or end-joining. Among end-joining mechanisms, the main process is classical non-homologous end-joining (C-NHEJ) which relies on Ku binding to DNA ends and DNA Ligase IV (Lig4)-mediated ligation. Mostly under Ku- or Lig4-defective conditions, an alternative end-joining process (A-EJ) can operate and exhibits a trend toward microhomology usage at the break junction. Homologous recombination relies on an initial MRN-dependent nucleolytic degradation of one strand at DNA ends. This process, named DNA resection generates 3' single-stranded tails necessary for homologous pairing with the sister chromatid. While it is believed from the current literature that the balance between joining and recombination processes at DSBs ends is mainly dependent on the initiation of resection, it has also been shown that MRN activity can generate short single-stranded DNA oligonucleotides (ssO) that may also be implicated in repair regulation. Here, we evaluate the effect of ssO on end-joining at DSB sites both in vitro and in cells. We report that under both conditions, ssO inhibit C-NHEJ through binding to Ku and favor repair by th...Continue Reading

References

Dec 23, 1988·Nucleic Acids Research·S Spitzer, F Eckstein
Apr 12, 1996·The Journal of Biological Chemistry·D W Chan, S P Lees-Miller
Mar 7, 1998·Biochemistry·S Yoo, W S Dynan
Jul 3, 1999·The Journal of Biological Chemistry·S YooW S Dynan
Jan 15, 2000·The Journal of Biological Chemistry·O HammarstenG Chu
Jan 11, 2005·Nucleic Acids Research·Katherine S PawelczakJohn J Turchi
Nov 8, 2006·Nucleic Acids Research·Minli WangGeorge Iliakis
Nov 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Pierre-Olivier MariDik C van Gent
Dec 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Josée Guirouilh-BarbatBernard S Lopez
Sep 24, 2008·Trends in Genetics : TIG·Mitch McVey, Sang Eun Lee
Nov 5, 2008·Methods in Molecular Biology·Sean P RyderJames R Williamson
Dec 27, 2008·The International Journal of Biochemistry & Cell Biology·Elzbieta PastwaThomas A Winters
Apr 15, 2009·The Journal of Experimental Medicine·Isabelle RobertBernardo Reina-San-Martin
Apr 22, 2010·The EMBO Journal·Oriane BombardePatrick Calsou
May 11, 2012·The Journal of Biological Chemistry·Michael G KempAziz Sancar
Jun 12, 2012·Nature Structural & Molecular Biology·Ammar A E AliAntony W Oliver
Aug 28, 2012·Molecular Cell·J Ross ChapmanSimon J Boulton
Apr 9, 2013·Frontiers in Genetics·Anabelle Decottignies
Apr 24, 2013·Proceedings of the National Academy of Sciences of the United States of America·Lan N TruongXiaohua Wu
Sep 5, 2013·Cold Spring Harbor Perspectives in Biology·Alexandre Maréchal, Lee Zou
Sep 21, 2013·Annual Review of Genetics·Ludovic Deriano, David B Roth
Jan 24, 2014·PLoS Genetics·Mireille BétermierBernard S Lopez
Feb 28, 2014·Nature Communications·Alejandro Álvarez-QuilónFelipe Cortés-Ledesma
Mar 13, 2014·DNA Repair·Philippe FritPatrick Calsou

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Citations

Feb 9, 2018·International Journal of Molecular Sciences·Kez ClealDuncan Baird
Jun 7, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yujing HuangQiao Cheng
May 1, 2021·International Journal of Molecular Sciences·Sayma ZahidJean Baptiste Charbonnier

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Methods Mentioned

BETA
electrophoretic mobility shift
pull-down
PCR
gel filtration
electrophoresis
flow cytometry
Electrophoretic mobility shift assay
transfection
footprinting

Software Mentioned

ssO
ImageJ
Exiqon
PRISM

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