Single-stranded RNA recognition by the bacteriophage T4 translational repressor, regA.

The Journal of Biological Chemistry
D BrownP Allen

Abstract

The T4 protein, RegA, is a translational repressor that blocks ribosome binding to multiple T4 messages by interacting with the mRNAs near their respective AUG start codons. Other than the AUG, there are no obvious similarities between the affected mRNAs. High affinity RNA ligands to RegA were isolated using SELEX (systematic evolution of ligands by exponential enrichment). The selected RNAs exhibited the consensus sequence 5'-AAAAUUGUUAUGUAA-3'. The AUG was invariant, suggesting that it is the primary effector of binding specificity. The UU immediately 5' to the AUG and the upstream poly(A) tract were highly conserved among the selected RNAs. Boundary and footprinting experiments are consistent with the consensus sequence defining the RegA-binding site. Interestingly, chemical modification and nuclease digestion data indicate that the RNA-binding site is single-stranded, as if RegA discriminates between targets based on their primary sequence, not their secondary structure. Minor variations from the consensus at positions other than the universally conserved AUG have little effect on RegA binding, but accumulation of mutations has a profound effect on the interaction. Comparison of the in vivo targets for RegA to the SELEX-gen...Continue Reading

References

Jan 1, 1992·Annual Review of Cell Biology·M Kozak
Jan 1, 1991·Methods in Enzymology·J Carey
Mar 1, 1990·Trends in Genetics : TIG·J E McCarthy, C Gualerzi
Jun 20, 1990·Journal of Molecular Biology·C TuerkL Gold
Dec 11, 1989·Nucleic Acids Research·K R WebsterE K Spicer
Jan 1, 1988·Annual Review of Biochemistry·L Gold
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R B WinterJ Karam
Nov 11, 1987·Nucleic Acids Research·J F MilliganO C Uhlenbeck
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J KaramM Dawson
Aug 25, 1995·Nucleic Acids Research·J BinkleyL Gold
Jan 1, 1994·Biochimie·N Standart, R J Jackson
Mar 10, 1995·The Journal of Biological Chemistry·S M O'MalleyE K Spicer

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Citations

Jul 31, 2012·Amino Acids·Yu-Hang Cai, He Huang
Aug 6, 1998·Current Opinion in Chemical Biology·M Famulok, A Jenne
Mar 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Eric S MillerWolfgang Rüger
Apr 24, 2009·Annual Review of Microbiology·Paul BabitzkeTony Romeo
Jul 14, 2007·Biomolecular Engineering·Regina StoltenburgBeate Strehlitz
May 4, 2005·Virology·Timothy R DeanEric S Miller
Sep 30, 2017·Molecular Biology and Evolution·Liam Abrahams, Laurence D Hurst
Jun 10, 2003·The Journal of Biological Chemistry·Kostas LymperopoulosPolly Roy
Jun 25, 1998·Molecular and Cellular Biology·H KanamoriD J Shapiro

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