Single stream inertial focusing in a straight microchannel

Lab on a Chip
Xiao WangIan Papautsky

Abstract

In the past two decades, microfluidics has become of great value in precisely aligning cells or microparticles within fluids. Microfluidic techniques use either external forces or sheath flow to focus particulate samples, and face the challenges of complex instrumentation design and limited throughput. The burgeoning field of inertial microfluidics brings single-position focusing functionality at throughput orders of magnitude higher than previously available. However, most inertial microfluidic focusers rely on cross-sectional flow-induced drag force to achieve single-position focusing, which inevitably complicates the device design and operation. In this work, we present an inertial microfluidic focuser that uses inertial lift force as the only driving force to focus microparticles into a single position. We demonstrate single-position focusing of different sized microbeads and cells with 95-100% efficiency, without the need for secondary flow, sheath flow or external forces. We further integrate this device with a laser counting system to form a sheathless flow cytometer, and demonstrated counting of microbeads with 2200 beads s(-1) throughput and 7% coefficient of variation. Cells can be completely recovered and remain viab...Continue Reading

References

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Citations

Sep 15, 2016·Sensors·Jingjing Zhao, Zheng You
Dec 20, 2016·Scientific Reports·Arzu ÖzbeyAli Koşar
Jan 20, 2017·Lab on a Chip·Jitao ZhangGiuliano Scarcelli
Sep 23, 2018·Electrophoresis·Yanli GongHai Jiang
Jul 20, 2019·The Analyst·Byeong Seon ParkBong Geun Chung
Mar 14, 2020·Micromachines·Amanda BogsethIan Papautsky
Dec 14, 2019·APL Bioengineering·Jian ZhouIan Papautsky
Sep 11, 2020·Lab on a Chip·Wenlai TangNan Xiang
Aug 17, 2020·Scientific Reports·Duncan P RyanHari S Viswanathan
Oct 21, 2020·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Yong HanZheng You

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