Single tube optimisation of APOE genotyping based on melting curve analysis

Clinical Biochemistry
Francesc FrancésDolores Corella

Abstract

To develop and validate a single-tube protocol for APOE genotyping using fluorescent probes. We have designed and validated a hybrid, single-tube, SimpleProbe/FRET probe protocol that takes advantage of the different probe wavelength emissions. Our method offers high quality outcomes, minimum interferences between probe signals and is 100% concordant with the reference protocol. This method is cheaper, faster and more reliable and versatile than other alternatives proposed.

References

Dec 30, 1988·Biochemical and Biophysical Research Communications·K H WeisgraberR W Mahley

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Citations

Mar 20, 2014·Genes & Nutrition·Elena H Martínez-LapiscinaMiguel A Martinez-Gonzalez
Mar 25, 2014·Pathology Oncology Research : POR·Péter Becságh, Orsolya Szakács
Sep 8, 2010·Clinica Chimica Acta; International Journal of Clinical Chemistry·Lu ZhengNing Xu

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