Single-walled and multi-walled carbon nanotubes induce sequence-specific epigenetic alterations in 16 HBE cells

Oncotarget
Manosij GhoshPeter H M Hoet

Abstract

Recent studies have identified carbon nanotube (CNT)-induced epigenetic changes as one of the key players in patho-physiological response. In the present study, we investigated whether CNT exposure is associated with epigenetic changes in human bronchial epithelial cells (16 HBE), in vitro. We focused on global DNA methylation, methylation of LINE-1 elements and promoter sequence of twelve functionally important genes (SKI, DNMT1, HDAC4, NPAT, ATM, BCL2L11, MAP3K10, PIK3R2, MYO1C, TCF3, FGFR 1 and AGRN). Additionally, we studied the influence of CNT exposure on miRNA expression. Using a LC-MS/MS method and pyrosequencing for LINE-1, we observed no significant changes in global DNA methylation (%) between the concentrations of multi-walled and single-walled CNT (MWCNT and SWCNT, respectively). Significant changes in sequence-specific methylation was observed in at least one CpG site for DNMT1 (SWCNT), HDAC4 (MWCNT), NPAT/ATM (MWCNT and SWCNT), MAP3K10 (MWCNT), PIK3R2 (MWCNT and SWCNT) and MYO1C (SWCNT). While changes in DNA methylation of the genes were relatively small, these changes were associated with changes in RNA expression, especially for MWCNT. However, the study did not reveal any significant alteration in the miRNA ex...Continue Reading

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Citations

Oct 17, 2019·Frontiers in Bioengineering and Biotechnology·Mallikarjuna Rao GeddaPrasoon Madhukar
Jan 8, 2021·Journal of Nanobiotechnology·Marta Pogribna, George Hammons
Apr 4, 2021·Cancers·Marcella Barbarino, Antonio Giordano
Apr 17, 2019·Chemical Research in Toxicology·Esra EmerceLode Godderis

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Methods Mentioned

BETA
Transmission electron microscopy
FCS
PCR

Software Mentioned

GraphPad
TopHat
EdgeR16
GraphPad Prism
Bowtie
PyroMark analysis
QuantStudio

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