Single X-ray irradiation modulates proteoglycan expression in brain tissue: investigation using mouse model.

Molecular Biology Reports
Maxim O PolitkoElvira V Grigorieva

Abstract

Radiotherapy is an integral part of glioblastoma treatment affecting both cancer cells and tumour microenvironment, where proteoglycans (PGs) are key extracellular components. However, the molecular effects of radiotherapy on PGs expression and functional activity in brain tissue are poorly understood. Here, we aimed to study the short-term effects of X-ray irradiation on PGs expression in normal brain tissue in mouse model in vivo. Two-month-old male CBL/6Bl mice (n = 54) were used in this study, animals' brains were irradiated using either research synchrotron VEPP-4 or clinical linear accelerator ElektaAxesse. Control (n = 18) and irradiated (n = 36) brain tissues were analysed at 24 h, 48 h and 72 h after irradiation. Morphology of the cortex and hippocampus was accessed by H&E staining, and expression of PGs (syndecan-1, glypican-1, HSPG2/perlecan, versican, brevican, neurocan, NG2/CSPG4, CD44, decorin, biglycan) was determined by RT-PCR. Single irradiation of mouse brain with a 7 Gy dose did not affect tissue morphology and mRNA levels of most highly-expressed PGs decorin and neurocan, although resulted in significant downregulation of brevican (3-10-fold) and NG2/CSPG4 (8-9-fold) expression both in cerebral cortex and su...Continue Reading

References

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Feb 23, 2020·International Journal of Molecular Sciences·Anastasia V SuhovskihElvira V Grigorieva

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Citations

Nov 3, 2020·Frontiers in Oncology·Elvira V Grigorieva

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Methods Mentioned

BETA
X-ray
light microscopy
RNA
PCR

Software Mentioned

ElektaAxesse
ImagePro6
OriginPro

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