Singlet-filtered NMR spectroscopy

Science Advances
Salvatore MamoneStefan Glöggler

Abstract

Selectively studying parts of proteins and metabolites in tissue with nuclear magnetic resonance promises new insights into molecular structures or diagnostic approaches. Nuclear spin singlet states allow the selection of signals from chemical moieties of interest in proteins or metabolites while suppressing background signal. This selection process is based on the electron-mediated coupling between two nuclear spins and their difference in resonance frequency. We introduce a generalized and versatile pulsed NMR experiment that allows populating singlet states on a broad scale of coupling patterns. This approach allowed us to filter signals from proton pairs in the Alzheimer's disease-related b-amyloid 40 peptide and in metabolites in brain matter. In particular, for glutamine/glutamate, we have discovered a long-lived state in tissue without the typically required singlet sustaining by radiofrequency irradiation. We believe that these findings will open up new opportunities to study metabolites with a view on future in vivo applications.

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Citations

Sep 2, 2020·NMR in Biomedicine·Salvatore MamoneStefan Glöggler
Dec 2, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·F TeleanuP R Vasos
Feb 27, 2021·Progress in Nuclear Magnetic Resonance Spectroscopy·Florin TeleanuPaul R Vasos
Apr 29, 2021·Physical Chemistry Chemical Physics : PCCP·Giulia MelchiorreGiuseppe Pileio
Jul 24, 2021·The Journal of Chemical Physics·Haolin ZhanZhong Chen
Aug 7, 2021·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Salvatore MamoneNasrollah Rezaei-Ghaleh

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Methods Mentioned

BETA
NMR
biosensors
total
dissecting

Software Mentioned

AVANCE

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