Singlet oxygen in Escherichia coli: New insights for antimicrobial photodynamic therapy

Free Radical Biology & Medicine
Xavier RagàsSanti Nonell

Abstract

Antimicrobial photodynamic therapy is an emerging treatment for bacterial infections that is becoming increasingly more attractive because of its effectiveness and unlikelihood of inducing bacterial resistance. However, there is limited knowledge about the localization of the photoactive drug in the bacteria and about the details of production of the main cytotoxic species, singlet oxygen. This article describes a combination of spectroscopic and time-resolved photophysical techniques that provide such information for a cationic porphyrin photosensitizer in gram-negative Escherichia coli bacteria. Our results reveal a double localization of the photosensitizer, inside (bound to the nucleic acids) and outside (bound to the cell wall) of the E. coli cells. Singlet oxygen is produced at both sites and is able to cross the cell wall.

References

Sep 1, 1987·Photochemistry and Photobiology·T A DahlP E Hartman
Jan 1, 1995·Journal of Medical Microbiology·M Wilson, C Yianni
Mar 1, 1994·Photochemistry and Photobiology·A Michaeli, J Feitelson
Sep 24, 1998·Journal of Photochemistry and Photobiology. B, Biology·Z SmetanaZ Malik
Sep 29, 2000·The Journal of Biological Chemistry·J L RavanatJ Cadet
Feb 28, 2002·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·M C TeichertM A Biel
Apr 1, 2003·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Anna SegallaElena Reddi
May 15, 2003·Chemical Reviews·Claude Schweitzer, Reinhard Schmidt
May 4, 2004·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Mali Salmon-DivonZvi Malik
May 4, 2004·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Michael R Hamblin, Tayyaba Hasan
May 4, 2005·Photochemistry and Photobiology·Mark J NiedreBrian C Wilson
Oct 20, 2005·Journal of the American Chemical Society·John W SnyderPeter R Ogilby
Oct 26, 2005·The Journal of Membrane Biology·G Stark
Jan 6, 2006·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Daniel A CaminosEdgardo N Durantini
Jun 22, 2006·Lasers in Surgery and Medicine·Giulio JoriGabrio Roncucci
Apr 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·Tim MaischWolfgang Bäumler
Apr 19, 2007·Current Medicinal Chemistry·J C StockertS Nonell
Dec 21, 2007·Journal of Photochemistry and Photobiology. B, Biology·Mariusz GrinholcKrzysztof P Bielawski
Mar 22, 2008·Free Radical Biology & Medicine·Ana Jiménez-BanzoSanti Nonell
Jul 29, 2008·Photochemistry and Photobiology·Sarika VermaTayyaba Hasan
Aug 30, 2008·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Ana Jiménez-BanzoSanti Nonell
May 19, 2009·Photodiagnosis and Photodynamic Therapy·Mariana B SpesiaEdgardo N Durantini
Jun 18, 2009·Lasers in Surgery and Medicine·S P TsengJ C Tsai
Nov 26, 2009·Photodiagnosis and Photodynamic Therapy·Mark Wainwright
Nov 26, 2009·Photodiagnosis and Photodynamic Therapy·Tianhong DaiMichael R Hamblin
Dec 17, 2009·Antimicrobial Agents and Chemotherapy·Francesco GiulianiGabrio Roncucci
Apr 1, 2009·Nature Chemistry·Marina K KuimovaPeter R Ogilby

❮ Previous
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Citations

Dec 1, 2010·Photochemistry and Photobiology·Threes G M Smijs, Stan Pavel
Feb 16, 2012·Photochemistry and Photobiology·Joana B PereiraJoão P C Tomé
Nov 22, 2012·Journal of Clinical Biochemistry and Nutrition·Hiroko Mori, Hideo Iwahashi
Jan 29, 2014·Future Medicinal Chemistry·Eliana AlvesAdelaide Almeida
May 9, 2012·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Patricia López-ChicónSanti Nonell
Jun 26, 2012·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Rubén Ruiz-GonzálezCristina Flors
Jan 17, 2013·Bioorganic & Medicinal Chemistry Letters·Li-Sheng ZhouMichael W Crowder
Jul 5, 2011·Free Radical Biology & Medicine·Orlando Chiarelli-NetoMaurício S Baptista
May 31, 2013·Bioorganic & Medicinal Chemistry·Eliana AlvesAdelaide Almeida
May 23, 2015·Future Microbiology·Milini ThomasLudmil T Benov
Sep 18, 2012·Journal of Photochemistry and Photobiology. B, Biology·R HariharanM Rajarajan
Jun 26, 2012·Journal of Colloid and Interface Science·Diógenes de Sousa Neto, Marcel Tabak
Aug 27, 2014·Physical Chemistry Chemical Physics : PCCP·Ariane FelgenträgerWolfgang Bäumler
Sep 14, 2012·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Maria C GomesÂngela Cunha
Oct 25, 2016·Methods : a Companion to Methods in Enzymology·Maria Garcia-DiazMichael R Hamblin
Oct 25, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Anna OniszczukKamila Kasprzak
Jan 23, 2016·Journal of Biophotonics·Anita GollmerCristina Flors
Mar 2, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xavier RagàsSanti Nonell
Apr 11, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Oriol PlanasSanti Nonell
May 12, 2018·Critical Reviews in Microbiology·Fabian CieplikTim Maisch
Oct 27, 2015·Scientific Reports·Pietro DelcanaleCristiano Viappiani
Jun 28, 2016·Experimental and Therapeutic Medicine·Y I LiangYou-Kun Lin
Sep 25, 2017·Photochemistry and Photobiology·Daniel B EcklHarald Huber
Apr 28, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Martha MerchánChristine Taviot-Guého
Dec 19, 2012·Israel Journal of Chemistry·Sulbha K SharmaMichael R Hamblin
Sep 13, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alex Fiori SilvaManuel Simões
Jan 15, 2021·Chemistry : a European Journal·Miguel Ángel Revuelta-MazaGema de la Torre
Jun 1, 2021·Frontiers in Medicine·Aleksandra Rapacka-ZdończykJoanna Nakonieczna

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