PMID: 2498913Apr 1, 1989Paper

Singlet oxygen involvement in the inactivation of cultured human fibroblasts by UVA (334 nm, 365 nm) and near-visible (405 nm) radiations

Photochemistry and Photobiology
R M Tyrrell, M Pidoux

Abstract

The UVA (320-380 nm) radiation inactivation of mammalian cells is dependent upon the presence of oxygen. In order to examine the intermediates involved, we have irradiated cells in the presence of chemical probes which are able to modify the activity of various oxygen species. We have also examined the possibility that UVA inactivates cultured human fibroblasts via generation of intracellular hydrogen peroxide. An iron scavenger (desferrioxamine) and a hydroxyl radical scavenger (dimethylsulfoxide) protect the cells against hydrogen peroxide. Diethyldithiocarbamate (a superoxide dismutase inhibitor) and aminotriazole (a catalase inhibitor) sensitize the cells to this oxidizing agent. These data support previous reports that hydrogen peroxide inactivates as a result of the iron-catalyzed generation of hydroxyl radical. None of these agents significantly alter the fluence-dependent inactivation of cell populations by radiation at 365 nm. In contrast, the cells are sensitized to radiation at 334, 365 and 405 nm in the presence of deuterium (an enhancer of singlet oxygen lifetime) and are protected against radiation at 365 nm by sodium azide (a quencher of singlet oxygen). These results are consistent with the conclusion that the g...Continue Reading

References

Apr 1, 1987·Molecular & General Genetics : MGG·A Eisenstark, G Perrot
Feb 15, 1984·The Biochemical Journal·A C Mello FilhoR Meneghini

❮ Previous
Next ❯

Citations

Mar 1, 2000·Biochemical and Biophysical Research Communications·H Yasui, H Sakurai
Sep 29, 2011·Archives of Dermatological Research·Yun Kyung LeeSungkwan An
Jul 30, 1991·Biochimica Et Biophysica Acta·P MorlièreL Dubertret
Apr 1, 1994·Toxicology in Vitro : an International Journal Published in Association with BIBRA·V MasiniJ Wepierre
May 1, 1995·Free Radical Biology & Medicine·R S Matthews
Sep 1, 1992·Mutation Research·H Sies, C F Menck
Mar 1, 1990·Journal of Photochemistry and Photobiology. B, Biology·R M Tyrrell, S M Keyse
Dec 1, 1992·Journal of Photochemistry and Photobiology. B, Biology·B S Rosenstein, R B Rosenstein
May 1, 1994·Journal of Photochemistry and Photobiology. B, Biology·B Bose, S N Chatterjee
May 1, 1995·Journal of Photochemistry and Photobiology. B, Biology·B Bose, S N Chatterjee
Feb 5, 2003·Free Radical Biology & Medicine·Marika C TrekliRex M Tyrrell
May 20, 1998·Free Radical Biology & Medicine·J Fuchs, J Thiele
Jun 26, 1998·Free Radical Biology & Medicine·S W Ryter, R M Tyrrell
Jan 12, 2001·Journal of Photochemistry and Photobiology. B, Biology·S E TobiT J McMillan
Oct 31, 2001·Journal of Photochemistry and Photobiology. B, Biology·A W Girotti
Aug 1, 1997·Journal of Photochemistry and Photobiology. B, Biology·H S BlackR M Tyrrell
Aug 1, 1997·Journal of Photochemistry and Photobiology. B, Biology·N EmonetM J Richard
Jan 24, 1998·Journal of Photochemistry and Photobiology. B, Biology·V ChekulayevA Kahru
Feb 1, 1993·British Journal of Haematology·M van Marwijk KooyH C van Prooijen
Jul 24, 2008·Journal of Clinical Biochemistry and Nutrition·Yoko AoSeiji Shioda
Sep 1, 1993·Current Eye Research·D SidjaninJ Reddan
Jul 27, 1999·The Journal of Investigative Dermatology·H MinamiT Tsuji
Mar 23, 2011·Journal of Photochemistry and Photobiology. B, Biology·Christian OpländerChristoph V Suschek
Aug 25, 2005·The Journal of Investigative Dermatology·Yu-Ying HeColin F Chignell
Feb 6, 2004·Journal of the European Academy of Dermatology and Venereology : JEADV·G MonfrecolaG Marone
Nov 30, 2007·Journal of Applied Microbiology·A HamamotoY Kinouchi
Jan 1, 1991·Chemico-biological Interactions·B Epe
Oct 7, 2006·Free Radical Biology & Medicine·Claudia von MontfortLars-Oliver Klotz

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.