PMID: 9165307Jan 1, 1997Paper

Singlet oxygen involvement in ultraviolet (254 nm) radiation-induced formation of 8-hydroxy-deoxyguanosine in DNA

Free Radical Biology & Medicine
H WeiX Zhang

Abstract

In the present article, we report that ultraviolet (UV 254 nm) radiation substantially induced the formation of 8-hydroxy-2'-deoxyguanosine (8-OHdG) in purified DNA. The formation of 8-OHdG, a hallmarker of oxidative DNA damage, increased linearly up to 25 kJ/m2 and was dependent on the presence of oxygen in the solution. Deoxygenation by nitrogen significantly reduced the yield of 8-OHdG by UV radiation, whereas oxygenation with 100% oxygen substantially enhanced the yield. The hydroxyl radical (HO.) scavenger dimethysulfoxide (DMSO) dramatically quenched the formation of 8-OHdG by the ionizing radiation and Fenton reaction, but enhanced the formation of UV-induced 8-OHdG. Further studies showed that DMSO and mannitol, two predominant HO. scavengers, enhanced the levels of UV-induced 8-OHdG in a dose-dependent fashion, suggesting that UV-induced 8-OHdG is independent of the generation of HO.. The use of deuterium oxide (D2O), which prolongs the half life of singlet oxygen (1O2), substantially enhanced the yield of 8-OHdG by UV radiation, but not that by Fenton reaction. In contrast, sodium azide, a more and less specific 1O2 quencher, substantially reduced the levels of 8-OHdG by both UV radiation and Fenton reaction, indicati...Continue Reading

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Citations

Oct 19, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Jenny Arnold, Tilman Grune
Mar 29, 2006·Journal of Plant Research·Kaori WatanabeYuichi Takeuchi
Oct 4, 2002·Archives of Biochemistry and Biophysics·Javier I OttavianiCesar G Fraga
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Sep 20, 2016·The Journal of Physical Chemistry Letters·Miguel Gomez-MendozaJean-Luc Ravanat
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