Sinomenine exerts anticonvulsant profile and neuroprotective activity in pentylenetetrazole kindled rats: involvement of inhibition of NLRP1 inflammasome

Journal of Neuroinflammation
Bo GaoWen-Ning Wu

Abstract

Epilepsy is a common neurological disorder and is not well controlled by available antiepileptic drugs (AEDs). Inflammation is considered to be a critical factor in the pathophysiology of epilepsy. Sinomenine (SN), a bioactive alkaloid with anti-inflammatory effect, exerts neuroprotective activity in many nervous system diseases. However, little is known about the effect of SN on epilepsy. The chronic epilepsy model was established by pentylenetetrazole (PTZ) kindling. Morris water maze (MWM) was used to test spatial learning and memory ability. H.E. staining and Hoechst 33258 staining were used to evaluate hippocampal neuronal damage. The expression of nucleotide oligomerization domain (NOD)-like receptor protein 1 (NLRP1) inflammasome complexes and the level of inflammatory cytokines were determined by western blot, quantitative real-time PCR and enzyme-linked immunosorbent assay (ELISA) kits. SN (20, 40, and 80 mg/kg) dose-dependently disrupts the kindling acquisition process, which decreases the seizure scores and the incidence of fully kindling. SN also increases the latency of seizure and decreases the duration of seizure in fully kindled rats. In addition, different doses of SN block the hippocampal neuronal damage and m...Continue Reading

References

Apr 30, 1991·Biochemical and Biophysical Research Communications·M MinamiM Satoh
Mar 1, 1993·Journal of Neurochemistry·H SchröderB Lössner
Aug 18, 2000·The European Journal of Neuroscience·M G De SimoniA Vezzani
Jun 11, 2004·Nature·Sanjeev MariathasanVishva M Dixit
Jun 23, 2004·Nature Reviews. Neuroscience·Michael A Rogawski, Wolfgang Löscher
Sep 29, 2004·Proceedings of the National Academy of Sciences of the United States of America·Nicola J BroadbentRobert E Clark
Nov 24, 2005·Epilepsia·Annamaria Vezzani, Tiziana Granata
Apr 4, 2006·Lancet·John S DuncanMatthew C Walker
Aug 22, 2006·Experimental Neurology·Akitaka YamamotoDavid C Henshall
Jun 29, 2007·Hippocampus·Véronique D Bohbot, Suzanne Corkin
Jul 10, 2007·Current Neurology and Neuroscience Reports·Helen E Scharfman
Sep 12, 2008·Epilepsy Research·S SinhaP Satishchandra
Dec 11, 2008·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Denise P AbulafiaW Dalton Dietrich
Jan 23, 2009·Nature·Teresa Fernandes-AlnemriEmad S Alnemri
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Edward A MiaoAlan Aderem
Mar 23, 2010·Cell·Kate Schroder, Jurg Tschopp
Dec 8, 2010·Nature Reviews. Neurology·Annamaria VezzaniTallie Z Baram
Sep 16, 2011·Journal of Neuroinflammation·Shilpa Mishra Shukla, Shiv K Sharma
Oct 12, 2011·Experimental Neurology·Annamaria VezzaniQuentin J Pittman
Oct 19, 2011·Biomarkers in Medicine·Annamaria Vezzani, Alon Friedman
Dec 14, 2011·Therapeutic Advances in Neurological Disorders·Clare M EddyAndrea E Cavanna
Jan 20, 2012·Nature·Till StrowigRichard Flavell
Apr 24, 2012·Neuropharmacology·Annamaria VezzaniRaymond J Dingledine
Apr 30, 2013·Glia·Julia MinkiewiczRobert W Keane
May 25, 2013·Nature Reviews. Immunology·Eicke LatzAndrea Stutz
Sep 21, 2013·Nature Reviews. Drug Discovery·Wolfgang LöscherDieter Schmidt
Jan 2, 2014·Frontiers in Immunology·Marcel R de Zoete, Richard A Flavell
Feb 25, 2014·Ageing Research Reviews·Li Liu, Christina Chan
Oct 24, 2014·Frontiers in Neuroscience·Gaurav SinghalBernhard T Baune
Feb 19, 2016·Archives of Physiology and Biochemistry·Suliman Y AlomarPaul Trayhurn
Mar 13, 2016·Free Radical Biology & Medicine·Xinjian ZhuHonghong Yao

❮ Previous
Next ❯

Citations

Sep 15, 2019·Neurochemical Research·Dong-Hai LiuJin-Ling Zhu
Jun 10, 2020·Journal of Neuroinflammation·Ao-Qi SongWen-Ning Wu
Apr 22, 2020·Journal of Neuroinflammation·Jun-Juan FanWen-Ning Wu
Apr 8, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Sicong LiLei Bi
Mar 20, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Menizibeya O Welcome, Nikos E Mastorakis
May 9, 2021·Brain Research Bulletin·Parvaneh Mohseni-MoghaddamCarlo Sala

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence microscopy
protein assay
PCR
ELISA

Software Mentioned

SPSS

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here