Abstract
Sinomenine (SIN) is an alkaloid found in the roots and stems of Sinomenium acutum, which has been used to treat rheumatic arthritis in China and Japan. In this study we investigated the effects of SIN on osteoclast survival in vitro and the mechanisms of the actions. Mature osteoclasts were differentiated from murine monocyte/macrophage cell line RAW264.7 through incubation in the presence of receptor activator of NF-κB ligand (RANKL, 100 ng/mL) for 4 d. The cell viability was detected using the CCK-8 method. The survival and actin ring construction of the osteoclasts were scored using TRACP staining and phalloidin-FITC staining, respectively. The apoptosis of the osteoclasts was detected by DNA fragmentation and Hoechst 33258 staining, and the cell necrosis was indicated by LDH activity. The activation of caspase-3 in osteoclasts was measured using Western blotting and the caspase-3 activity colorimetric method. SIN (0.25-2 mmol/L) inhibited the viability of mature osteoclasts in dose-dependent and time-dependent manners, but did not affect that of RAW264.7 cells. Consistently, SIN dose-dependently suppressed the survival of mature osteoclasts. The formation of actin ring, a marker associated with actively resorbing osteoclast...Continue Reading
References
Jun 1, 1989·The Anatomical Record·R Baron
Dec 1, 1999·Cell Death and Differentiation·D W Nicholson
Dec 26, 2001·Pharmacology & Therapeutics·K C ZimmermannD R Green
May 16, 2003·Nature·William J BoyleDavid L Lacey
Feb 8, 2005·Biochemical and Biophysical Research Communications·Lianping Xing, Brendan F Boyce
Mar 26, 2005·Immunology Letters·Xiaobo HeXuetao Cao
Aug 23, 2005·Biochemical and Biophysical Research Communications·Guoliang GuH Kalervo Väänänen
Oct 11, 2005·Biochemical and Biophysical Research Communications·Yun Ha HwangChul-Hak Yang
Jul 13, 2006·Annals of the New York Academy of Sciences·Sakae TanakaKozo Nakamura
Sep 15, 2006·Journal of Cellular Physiology·K H SzymczykM J Steinbeck
Feb 13, 2007·Biochemical and Biophysical Research Communications·Takayuki YonezawaJe-Tae Woo
Mar 8, 2007·Annals of the New York Academy of Sciences·Roberta PivaRoberto Gambari
Mar 27, 2007·Cell Biology International·Luan ShuZi-Chun Hua
Apr 5, 2007·Journal of Cellular Biochemistry·Paula H Stern
Aug 7, 2008·Planta medica·Min XuXiong Cai
Oct 1, 2008·Biomedical Materials·Xia LiAtsushi Yamazaki
Nov 13, 2008·Molecular Cancer Therapeutics·Toru AkiyamaPeter F M Choong
Aug 5, 2009·Journal of Cellular Physiology·Myung Hee KimSeong Hwan Kim
Sep 3, 2009·Annals of the New York Academy of Sciences·Roberta PivaRoberto Gambari
Sep 23, 2011·Journal of Cellular Physiology·Tetyana ShandalaCory J Xian
Feb 7, 2012·Molecular Medicine Reports·Jun ZhouYan Zhang
Jun 20, 2012·Current Osteoporosis Reports·Pierre J Marie
Jul 11, 2012·Pharmaceutical Biology·Xiang-Xiang ZhaoLu-Ping Qin
Aug 1, 2012·Oncology Letters·Yifei LvZhiming Hao
Nov 21, 2012·International Journal of Oncology·Xin-Lan LuShui-Χiang He
Citations
Nov 26, 2015·Acta Pharmacologica Sinica·Xiang-zhou ZengShu-wen Liu
Mar 12, 2016·European Journal of Pharmacology·Longgang HeXiaojuan Li
Nov 4, 2015·International Immunopharmacology·Zijian ZhaoDelie An
Jul 28, 2016·Cytotechnology·Travers SagarVishwa Deepak
Sep 25, 2016·Molecular and Cellular Endocrinology·Zhen TanJiake Xu
Nov 22, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zi-Jian ZhaoJian-Cheng Wang
Jun 28, 2019·Basic & Clinical Pharmacology & Toxicology·Haifeng YuanHaichao Wang
Nov 9, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Yuting XuFei Wang
Oct 27, 2018·Scientific Reports·Tahereh JamaliSusan K Ardestani
Nov 8, 2018·Experimental and Therapeutic Medicine·Hong-Tao WangJun Yao
Jun 9, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Shan-Shan LinXian-Liang Wang
Jul 18, 2019·Royal Society Open Science·Liuliu YanKun Wei
Oct 30, 2020·Frontiers in Pharmacology·Yingjie ShiXiaojuan He