Sinomenine Induces G1-Phase Cell Cycle Arrest and Apoptosis in Malignant Glioma Cells Via Downregulation of Sirtuin 1 and Induction of p53 Acetylation

Technology in Cancer Research & Treatment
Xiaoyan HeHongyan Li

Abstract

Sinomenine, a bioactive alkaloid isolated from the traditional Chinese herb Sinomenium acutum, possesses antiinflammatory, antinociceptive, antifibrotic, and antitumorigenic properties. In this work, we sought to explore the biological effects of sinomenine on glioma cells. It was found that sinomenine caused a concentration-dependent inhibition of viability in both U87 and U251 glioma cells. Sinomenine at 16 μmol/L caused 55% to 60% reduction in the proliferation of U87 and U251 cells. Moreover, sinomenine treatment induced a G0/G1 cell cycle arrest and apoptosis. Mechanistically, sinomenine promoted p53 expression and acetylation and reduced the expression of sirtuin 1. Ectopic expression of sirtuin 1 significantly prevented sinomenine-induced p53 acetylation and growth suppression in glioma cells. Moreover, sinomenine inhibited the growth of U87 xenograft tumors in vivo and raised the p53 protein expression. Collectively, sinomenine shows antiproliferative effects against glioma cells which is mediated through downregulation of sirtuin 1 and induction of p53 activity.

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Citations

May 3, 2019·Cell Death & Disease·Neelum Aziz YousafzaiXian Wang
May 28, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Guya Diletta MarconiSusi Zara
May 1, 2020·International Journal of Molecular Sciences·Kun-Hung ShenPin-Shern Chen
Dec 31, 2019·Seminars in Cancer Biology·Thomas Efferth, Franz Oesch

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
electrophoresis
Xenografts
flow cytometry
acetylation
xenograft

Software Mentioned

Quantity One

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