Sinorhizobium meliloti mutants deficient in phosphatidylserine decarboxylase accumulate phosphatidylserine and are strongly affected during symbiosis with alfalfa.

Journal of Bacteriology
Miguel Angel Vences-GuzmánChristian Sohlenkamp

Abstract

Sinorhizobium meliloti contains phosphatidylglycerol, cardiolipin, phosphatidylcholine, and phosphatidylethanolamine (PE) as major membrane lipids. PE is formed in two steps. In the first step, phosphatidylserine synthase (Pss) condenses serine with CDP-diglyceride to form phosphatidylserine (PS), and in the second step, PS is decarboxylated by phosphatidylserine decarboxylase (Psd) to form PE. In this study we identified the sinorhizobial psd gene coding for Psd. A sinorhizobial mutant deficient in psd is unable to form PE but accumulates the anionic phospholipid PS. Properties of PE-deficient mutants lacking either Pss or Psd were compared with those of the S. meliloti wild type. Whereas both PE-deficient mutants grew in a wild-type-like manner on many complex media, they were unable to grow on minimal medium containing high phosphate concentrations. Surprisingly, the psd-deficient mutant could grow on minimal medium containing low concentrations of inorganic phosphate, while the pss-deficient mutant could not. Addition of choline to the minimal medium rescued growth of the pss-deficient mutant, CS111, to some extent but inhibited growth of the psd-deficient mutant, MAV01. When the two distinct PE-deficient mutants were analy...Continue Reading

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Citations

Jun 14, 2013·BMC Microbiology·Louise Bélanger, Trevor C Charles
Apr 12, 2015·FEMS Microbiology Reviews·Christian Sohlenkamp, Otto Geiger
Mar 2, 2016·Biochimica Et Biophysica Acta·Meike SiebersPeter Dörmann
Apr 9, 2014·Molecular Microbiology·Roman MoserFranz Narberhaus
Feb 26, 2015·Environmental Microbiology·Diana X Sahonero-CanavesiOtto Geiger
Dec 25, 2009·FEMS Microbiology Letters·Daniela B MedeotIsabel M López-Lara
Sep 12, 2018·Canadian Journal of Microbiology·George C diCenzoAlessio Mengoni
Aug 26, 2009·Progress in Lipid Research·Otto GeigerChristian Sohlenkamp
Aug 10, 2021·Frontiers in Plant Science·Otto GeigerIsabel M López-Lara

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