Sinusoid development and morphogenesis may be stimulated by VEGF-Flk-1 signaling during fetal mouse liver development

Developmental Dynamics : an Official Publication of the American Association of Anatomists
Yoshinori SugiyamaNobuyoshi Shiojiri

Abstract

Early morphogenesis of hepatic sinusoids was histochemically and experimentally analyzed, and the importance of VEGF-Flk-1 signaling in the vascular development was examined during murine liver organogenesis. FITC-gelatin injection experiments into young murine fetuses demonstrated that all primitive sinusoidal structures were confluent with portal and central veins, suggesting that hepatic vessel development may occur via angiogenesis. At 12.5-14.5 days of gestation, VEGF receptors designated Flk-1, especially their mature form, were highly expressed in endothelial cells of primitive sinusoidal structures and highly phosphorylated on their tyrosine residues. At the same time, VEGF was also detected in hepatoblasts/hepatocytes, hemopoietic cells, and megakaryocytes of the whole liver parenchyma. Furthermore, the addition of VEGF to E12.5 liver cell cultures significantly induced the growth and branching morphogenesis of sinusoidal endothelial cells. Therefore, VEGF-Flk-1 signaling may play an important role in the growth and morphogenesis of primitive sinusoids during fetal liver development.

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Citations

May 23, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Natalia SerranoMaría Luisa Gaspar
Jun 26, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Cyrill GéraudSergij Goerdt
Feb 4, 2014·Organogenesis·Yoshie KadotaYuko Kitagawa
Nov 2, 2014·Stem Cell Reports·Orit GoldmanValerie Gouon-Evans
Jul 18, 2016·Journal of Hepatology·Johanne PoissonPierre-Emmanuel Rautou
Nov 5, 2016·Reproductive Toxicology·John M DeSesso
Aug 15, 2020·Frontiers in Cell and Developmental Biology·Francisca Soares-da-SilvaPerpetua Pinto-do-Ó
Dec 24, 2010·Current Opinion in Endocrinology, Diabetes, and Obesity

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