Sinusoidal endothelial cell injury by superoxide anion and iron in the Propionibacterium acnes-pretreated and lipopolysaccharide-stimulated rat liver

Liver
T HasegawaHiroyuki Kuwano

Abstract

We attempted to measure the generation of superoxide anion, examine its site of release and determine its pathological role in Propionibacterium acnes-lipopolysaccharide-induced liver injury in the rat. The P. acnes-pretreated (16 mg/kg i.v.) rat liver was perfused with buffer containing lipopolysaccharide (2.5 microg/ml). Chemiluminescence enhanced with Cypridina luciferin analog, MCLA, and reduction of nitro blue tetrazolium were used for detecting superoxide anion. Leakage of enzymes and release of cytokines into the perfusate, and histological specimens were also examined. Superoxide dismutase-inhibitable chemiluminescence peaked at 30 min of lipopolysaccharide infusion and blue formazan precipitate was histochemically deposited mainly on hepatic macrophages. Purine nucleoside phosphorylase (PNP) activity in the perfusate, as a marker of sinusoidal endothelial cell injury, reached its maximum at 50 min and aspartate aminotransferase (AST) activity, as a marker of hepatocyte injury, reached a plateau at 90 min. Simultaneous treatment with superoxide dismutase and deferoxamine mesylate significantly suppressed the leakage of PNP and AST. Release of tumor necrosis factor-alpha and growth-related oncogene/cytokine-induced neutr...Continue Reading

References

Jan 1, 1978·Methods in Enzymology·P A HoffeeB D Robertson
Jan 1, 1992·Free Radical Biology & Medicine·M OkudaC Kumar
Jul 27, 1992·FEBS Letters·M Baggiolini, I Clark-Lewis
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J S BeckmanB A Freeman
Nov 1, 1990·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J W Larrick, S C Wright
Feb 9, 1989·The New England Journal of Medicine·S J Weiss
Jan 1, 1985·Gastroenterology·G M AndersonD J Cohen
Nov 1, 1985·Gastroenterology·M J ArthurR Wright
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·B FreiB N Ames
Sep 1, 1973·Microvascular Research·A M Rappaport
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·A BoverisB Chance
Jan 1, 1995·Scandinavian Journal of Gastroenterology·I SakaidaK Okita
Oct 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·B G HarbrechtT R Billiar
Nov 15, 1994·Transplantation·J C GerlachP Neuhaus
Apr 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H JaeschkeC W Smith
Feb 1, 1996·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S MochidaK Fujiwara
Jul 1, 1996·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S SuzukiS Baba
Jul 1, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H ShibuyaS Satomi

❮ Previous
Next ❯

Citations

Oct 2, 2003·Biological & Pharmaceutical Bulletin·Hiroshi KuriharaXin-Sheng Yao
Apr 4, 2021·Journal of Gastroenterology and Hepatology·Yang Wang, Yulan Liu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.