SIRB, sans iron oxide rhodamine B, a novel cross-linked dextran nanoparticle, labels human neuroprogenitor and SH-SY5Y neuroblastoma cells and serves as a USPIO cell labeling control

Contrast Media & Molecular Imaging
Wei-Bin ShenPaul Yarowsky

Abstract

This is the first report of the synthesis of a new nanoparticle, sans iron oxide rhodamine B (SIRB), an example of a new class of nanoparticles. SIRB is designed to provide all of the cell labeling properties of the ultrasmall superparamagnetic iron oxide (USPIO) nanoparticle Molday ION Rhodamine B (MIRB) without containing the iron oxide core. MIRB was developed to label cells and allow them to be tracked by MRI or to be manipulated by magnetic gradients. SIRB possesses a similar size, charge and cross-linked dextran coating as MIRB. Of great interest is understanding the biological and physiological changes in cells after they are labeled with a USPIO. Whether these effects are due to the iron oxide buried within the nanoparticle or to the surface coating surrounding the iron oxide core has not been considered previously. MIRB and SIRB represent an ideal pairing of nanoparticles to identify nanoparticle anatomy responsible for post-labeling cytotoxicity. Here we report the effects of SIRB labeling on the SH-SY5Y neuroblastoma cell line and primary human neuroprogenitor cells (hNPCs). These effects are contrasted with the effects of labeling SH-SY5Y cells and hNPCs with MIRB. We find that SIRB labeling, like MIRB labeling, (i)...Continue Reading

References

Apr 1, 2004·Human Gene Therapy·Ali S ArbabJoseph A Frank
May 8, 2004·Biomaterials·Catherine Cecilia BerryAdam S G Curtis
Feb 27, 2007·Biomaterials·Thomas R PisanicSungho Jin
Apr 14, 2009·Methods : a Companion to Methods in Enzymology·Uwe Himmelreich, Tom Dresselaers
Sep 29, 2009·Colloids and Surfaces. B, Biointerfaces·Morteza MahmoudiPieter Stroeve
Dec 7, 2011·Contrast Media & Molecular Imaging·C McFaddenP J Foster
Dec 27, 2011·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Kirsten MessmerPaul S Fishman
Feb 18, 2012·Annual Review of Physiology·Joseph A Mindell
Apr 15, 2014·International Journal of Nanomedicine·Elizabeth BullAlexander M Seifalian

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