SIRF--a novel regulator element controlling transcription from the p55Cdc/Fizzy promoter during the cell cycle

Biochemical and Biophysical Research Communications
Ulrike HaugwitzKurt Engeland

Abstract

p55Cdc proteins participate in activation and timing of ubiquitin ligation by APC/C. Labeling of the substrates with ubiquitin leads to degradation of the cell cycle proteins through the proteasome in mitosis. Consistent with the phase in which the protein functions p55Cdc mRNA is expressed during the cell cycle starting in S phase with a maximum in G2/M. We analyzed the human p55Cdc promoter responsible for this expression pattern and found with SIRF (Cell-Cycle Site-Regulating p55Cdc/Fizzy-Transcription) a novel element which downregulates transcription in a cell cycle-dependent manner. Activation of gene transcription is independent of the SIRF element and NF-Y. The nucleotide sequence of SIRF is essentially identical in human, rat, and mouse p55Cdc whereas other parts of the promoter are not conserved. SIRF requires its natural promoter context for its regulatory function. With a length of 44 nucleotides this element is unusually long and may require a large protein complex for its regulation.

References

Mar 15, 1996·Science·J ZwickerR Müller
Jun 15, 1995·The Biochemical Journal·J Pines
Dec 6, 1996·Science·K Nasmyth
Apr 4, 1998·Nucleic Acids Research·R Mantovani
Jul 31, 1998·Molecular Genetics and Metabolism·J WeinsteinK M Sakamoto
Aug 8, 1998·Genes & Development·N Dyson
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·K E BoydP J Farnham
Jun 25, 1999·Biochemical and Biophysical Research Communications·M ShteinbergA Hershko
Nov 13, 1999·Nature Cell Biology·P Clute, J Pines
Mar 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Julie WellsPeggy J Farnham
Jun 7, 2002·Molecular Cell·Jan-Michael Peters
Dec 11, 2002·Current Opinion in Cell Biology·Olivier Stevaux, Nicholas J Dyson
Dec 17, 2002·The Journal of Biological Chemistry·Valentina SalsiRoberto Mantovani

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Citations

Mar 21, 2007·Journal of Biomedical Science·Rey-Huei Chen
Apr 21, 2005·Seminars in Cell & Developmental Biology·Tsz Kan Fung, Randy Y C Poon
Nov 11, 2017·Cell Death and Differentiation·Kurt Engeland
Jan 1, 2018·Cell Death and Differentiation·Kurt Engeland

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