siRNA delivery into tumor cells by cationic cholesterol derivative-based nanoparticles and liposomes

Biological & Pharmaceutical Bulletin
Yoshiyuki HattoriEtsuo Yonemochi

Abstract

Previously, we reported that cationic nanoparticles (NP) composed of diamine-type cholesteryl-3-carboxamide (OH-Chol, N-(2-(2-hydroxyethylamino)ethyl)cholesteryl-3-carboxamide) and Tween 80 could deliver small interfering RNA (siRNA) with high transfection efficiency into tumor cells. In this study, we synthesized new diamine-type cationic cholesteryl carbamate (OH-C-Chol, cholesteryl (2-((2-hydroxyethyl)amino)ethyl)carbamate) and triamine-type carbamate (OH-NC-Chol, cholesteryl (2-((2-((2-hydroxyethyl)amino)ethyl)amino)ethyl)carbamate), and prepared cationic nanoparticles composed of OH-C-Chol or OH-NC-Chol with Tween 80 (NP-C and NP-NC, respectively), as well as cationic liposomes composed of OH-C-Chol or OH-NC-Chol with 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) (LP-C and LP-NC, respectively) for evaluation of their possible use as siRNA delivery vectors. LP-C and LP-NC/siRNA complexes (lipoplexes) exhibited larger gene silencing effects than NP-C and NP-NC/siRNA complexes (nanoplexes), respectively, in human breast tumor MCF-7 cells, although the NP-C nanoplex showed high association with the cells. In particular, LP-NC lipoplex could induce strong gene suppression, even at a concentration of 5 nM siRNA. From thes...Continue Reading

References

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Sep 12, 2008·Bioorganic & Medicinal Chemistry Letters·Yoshiyuki HattoriYoshie Maitani
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Jan 3, 2013·International Journal of Pharmaceutics·Yoshiyuki HattoriYoshie Maitani
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Jan 5, 2014·Advanced Drug Delivery Reviews·Bulent OzpolatGabriel Lopez-Berestein

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Citations

Jun 28, 2016·Journal of Liposome Research·Yoshiyuki HattoriHiraku Onishi
Jul 3, 2020·Chemical & Pharmaceutical Bulletin·Shintaro Fumoto, Koyo Nishida
Jul 1, 2020·Frontiers in Bioengineering and Biotechnology·Zhenzhen ZhangXiang Zhou
Apr 6, 2021·Frontiers in Bioengineering and Biotechnology·Tulsi Ram DamaseJohn P Cooke
Jul 20, 2018·ACS Omega·Hung DoHamidreza Montazeri Aliabadi

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