siRNA high-throughput kinase library screen identifies protein kinase, DNA-activated catalytic polypeptide to play a role in MyD88-induced IFNA2 activation and IL-8 secretion

Biotechnology and Applied Biochemistry
Nikolaos CharlaftisPeter J Morley

Abstract

The discovery of RNA interference has led to the development of short interfering RNA (siRNA) screening, which has been widely used to study biological pathways. Here, we describe the development and validation of a system suitable to identify cellular genes involved in interferon A2 (IFNA2) promoter activation and interleukin (IL)-8 secretion downstream of MyD88. Forty genes were identified. Five genes were selected for further study. One gene, protein kinase, DNA-activated catalytic polypeptide (PRKDC), was confirmed to play a role in MyD88-induced IFNA2 activation and IL-8 secretion.

References

Mar 11, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hiroaki HemmiShizuo Akira
Oct 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·Kenya HondaTadatsugu Taniguchi
Dec 28, 2006·Journal of Biochemistry·Taro Kawai, Shizuo Akira
Nov 6, 2007·Immunological Reviews·Shen-Ying ZhangJean-Laurent Casanova
Nov 6, 2007·Immunological Reviews·Arthur M Krieg, Jörg Vollmer
Nov 22, 2007·Trends in Molecular Medicine·Taro Kawai, Shizuo Akira
Jan 8, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Satoshi YotsumotoYukihiko Aramaki
Apr 5, 2008·Drug News & Perspectives·Richard L MillerMark A Tomai
Jun 13, 2009·Journal of Translational Medicine·David O AzorsaSpyro Mousses
Aug 12, 2010·Methods in Enzymology·Christoph SchanielIhor R Lemischka
Aug 18, 2010·Combinatorial Chemistry & High Throughput Screening·Xiahui XiongMeihong Chen
Sep 23, 2010·Science Signaling·Igor AstsaturovErica A Golemis

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