Abstract
How to obtain a small interfering RNA (siRNA) vector has become a moot point in recent years. Exosomes (Exo) show advantages of long survival time in vivo, high transmission efficiency, and easy penetration across the blood-spinal cord barrier, renowned as excellent carriers of bioactive substances. We applied mesenchymal stem cell (MSC)-derived exosomes as the delivery of synthesized siRNA, which were extracted from rat bone marrow. We constructed exosomes-siRNA (Exo-siRNA) that could specifically silence CTGF gene in the injury sites by electroporation. During the administration, we injected Exo-siRNA into the tail vein of SCI rats, RESULTS: In vivo and in vitro experiments showed that Exo-siRNA not only effectively inhibited the expressions of CTGF gene, but quenched inflammation, and thwarted neuronal apoptosis and reactive astrocytes and glial scar formation. Besides, it significantly upregulated several neurotrophic factors and anti-inflammatory factors, acting as a facilitator of locomotor recovery of rats with spinal cord injury (SCI). In conclusion, this study has combined the thoroughness of gene therapy and the excellent drug-loading characteristics of Exo for the precise treatment of SCI, which will shed new light o...Continue Reading
References
Mar 31, 2005·Journal of Neurosurgery. Spine·Sabine ConradJan M Schwab
Dec 3, 2014·Glia·Xi WangYi Ren
Mar 12, 2016·Cell·Mercedes Tkach, Clotilde Théry
Sep 27, 2016·Cell Stem Cell·Thomas M FandelArnold R Kriegstein
Jan 17, 2017·The Spine Journal : Official Journal of the North American Spine Society·Yuan WangJian-Gang Shi
Jul 1, 2017·Journal of Neurotrauma·Jiang-Hu HuangFei-Yue Lin
Jul 12, 2018·Nano Letters·Han Young KimByung-Soo Kim
Jul 25, 2018·Journal of Molecular and Cellular Cardiology·Lisa E DornFederica Accornero
Sep 18, 2018·The Spine Journal : Official Journal of the North American Spine Society·Viktor Bartanusz, Naomi Sayre
Dec 14, 2018·Frontiers in Neuroscience·Dong LiXiaocheng Lu
Dec 26, 2018·Journal of Visualized Experiments : JoVE·Farid N FaruquKhuloud T Al-Jamal
Feb 1, 2019·Nature Reviews. Neurology·Zheng Gang ZhangMichael Chopp
Feb 15, 2019·Nano Letters·Nisim PeretsDaniel Offen
Feb 17, 2019·Cancer Letters·Farrukh AqilRamesh C Gupta
Apr 6, 2019·Cell·Dennis K JeppesenRobert J Coffey
Aug 23, 2019·Bone Marrow Transplantation·Mayela MendtElizabeth Shpall
Sep 4, 2019·ACS Nano·Golnaz MoradMarsha A Moses
Feb 8, 2020·Science·Raghu Kalluri, Valerie S LeBleu
Feb 12, 2020·Nature Reviews. Genetics·Chengwen Li, R Jude Samulski
May 20, 2020·Drug Design, Development and Therapy·Jun GuSheng Chen
Jun 2, 2020·Redox Biology·Katrin Schröder
Jun 11, 2020·Nature Reviews. Neuroscience·Itzhak FischerMichael A Lane
Oct 16, 2020·The Journal of Spinal Cord Medicine·Yukihide NishimuraFumihiro Tajima
Nov 8, 2020·Nature Biomedical Engineering·Shiyu LiuYan Jin
Nov 25, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Andrea RaspaFabrizio Gelain
Dec 4, 2020·International Immunology·Hiroshi Tsujioka, Toshihide Yamashita
Dec 15, 2020·Nature Reviews. Neurology·Karim FouadJan M Schwab
Dec 23, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Di WuZhengkai Wei
Feb 5, 2021·Stem Cell Research & Therapy·Wen-Zhao LiuXue-Wen Kang
Mar 7, 2021·Cell Stem Cell·Wenjiao TaiChun-Li Zhang