siRNA-mediated knock-down of NOX3: therapy for hearing loss?

Cellular and Molecular Life Sciences : CMLS
Leonard P RybakVickram Ramkumar

Abstract

Cisplatin is a widely used chemotherapeutic agent that causes significant hearing loss. Previous studies have shown that cisplatin exposure is associated with increase in reactive oxygen species (ROS) in the cochlea. The inner ear expresses a unique isoform of NADPH oxidase, NOX3. This enzyme may be the primary source of ROS generation in the cochlea. The knockdown of NOX3 by pretreatment with siRNA prevented cisplatin ototoxicity, as demonstrated by preservation of hearing thresholds and inner ear sensory cells. Trans-tympanic NOX3 siRNA reduced the expression of NOX3 and biomarkers of cochlear damage, including transient receptor vanilloid 1 (TRPV1) channel and kidney injury molecule-1 (KIM-1) in cochlear tissues. In addition, siRNA against NOX3 reduced apoptosis as demonstrated by TUNEL staining, and prevented the increased expression of Bax and abrogated the decrease in Bcl2 expression following cisplatin administration. Trans-tympanic administration of siRNA directed against NOX3 may provide a useful method of attenuating cisplatin ototoxicity. In this paper, we review recent publications dealing with the role of NOX3 in ototoxicity and the effects of siRNA against cisplatin-induced hearing loss.

References

Aug 1, 1975·Toxicology and Applied Pharmacology·R W FleischmanU Schaeppi
Apr 1, 1984·Environmental Health Perspectives·J S Bus, J E Gibson
Oct 1, 1995·Drug Safety : an International Journal of Medical Toxicology and Drug Experience·M J McKeage
Nov 27, 1999·Hearing Research·K C CampbellL F Hughes
Jul 5, 2001·Toxicology and Applied Pharmacology·N DehneH de Groot
Nov 6, 2003·Hearing Research·Steven SluyterGuido F Smoorenburg
Mar 12, 2004·Genes & Development·Rainer PaffenholzDavid E Bergstrom
Aug 19, 2004·ORL; Journal for Oto-rhino-laryngology and Its Related Specialties·Ji Eun LeeJuichi Ito
Aug 25, 2004·The Journal of Biological Chemistry·Botond BánfiKarl-Heinz Krause
Oct 7, 2004·Audiology & Neuro-otology·Thomas M NicoteraRichard Salvi
Oct 30, 2004·Acta Oto-laryngologica·Chong-Sun KimYoung Ho Kim
Nov 3, 2004·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Y LiJ H Silber
Jun 7, 2005·Hearing Research·Eric C BielefeldDonald Henderson
Feb 1, 2006·Ear and Hearing·Donald HendersonBo Hua Hu
Nov 23, 2006·Hearing Research·Leonard P RybakVickram Ramkumar
Sep 8, 2007·Current Opinion in Otolaryngology & Head and Neck Surgery·Ilaaf DarratMichael D Seidman
Jun 3, 2008·Toxicology·O'Neil W Guthrie
Dec 5, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Debashree MukherjeaVickram Ramkumar
May 20, 2009·Antioxidants & Redox Signaling·Ana Clara CristóvãoYoon-Seong Kim
Oct 24, 2009·The Tohoku Journal of Experimental Medicine·Leonard P RybakVickram Ramkumar
Dec 17, 2010·Mammalian Genome : Official Journal of the International Mammalian Genome Society·John P FlahertyDavid E Bergstrom

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Citations

Apr 18, 2013·Japanese Journal of Clinical Oncology·Keita UchinoFumitaka Takeshita
Nov 10, 2013·Biogerontology·Hengchao Chen, Jianguo Tang
Oct 14, 2014·Current Otorhinolaryngology Reports·Cameron C WickCliff A Megerian
Sep 23, 2014·Expert Opinion on Investigational Drugs·Debashree MukherjeaVickram Ramkumar
Feb 26, 2016·Antioxidants & Redox Signaling·Y I ZhangPatty J Lee
Aug 1, 2013·Hearing Research·Srdjan M VlajkovicPeter R Thorne
Jun 22, 2019·International Journal of Toxicology·David V GauvinTheodore J Baird
Nov 22, 2016·Journal of Cellular Physiology·Rahul MittalXue Zhong Liu
Jul 17, 2018·BioMed Research International·Min Yong Lee, Yong-Ho Park
Jul 29, 2020·Frontiers in Pharmacology·Dehong YuHao Wu
Aug 23, 2020·Free Radical Biology & Medicine·Ignacio A Cortés FuentesJuan G Gormaz

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