Abstract
To investigate the effects of small interference RNA (siRNA) targeting of Cdx2 on human gastric cancer MGC-803 cells in vitro and in vivo. The recombinant pSilencer 4.1-Cdx2 siRNA plasmids were constructed and transfected into gastric cancer MGC-803 cells in vitro. The stable transfectants were selected. The effects of Cdx2 siRNA on growth, proliferation, cell cycle, apoptosis, migration and invasiveness of human gastric cancer MGC-803 cells were evaluated and the expression of phosphatase and tensin homolog (PTEN), caspase-9 and caspase-3 was observed in vitro by reverse transcription polymerase chain reaction (RT-PCR) and Western blotting analysis. We also investigated the effect of Cdx2 siRNA on growth of MGC-803 cells in nude mice in vivo. Cdx2 siRNA led to inhibition of endogenous Cdx2 mRNA and protein expression as determined by RT-PCR and Western blotting analysis. Cdx2 siRNA significantly inhibited cell growth and proliferation, blocked entry into the S-phase of the cell cycle, induced cell apoptosis, and reduced the motility and invasion of MGC-803 cells. Cdx2 siRNA also increased PTEN expression, and activated caspase-9 and caspase-3 in MGC-803 cells in vitro . In addition, siRNA targeting of Cdx2 inhibited the growth...Continue Reading
References
Jan 16, 2002·Cancer Letters·Yun-Qing BaiYasuhito Yuasa
Dec 11, 2002·The Journal of Pathology·Raquel AlmeidaLeonor David
Sep 13, 2003·Gut·C BonhommeC Domon-Dell
Nov 20, 2003·Nature Genetics·Koji AokiMakoto M Taketo
May 12, 2004·Cancer Biology & Therapy·Rong-Jun GuoJohn P Lynch
Jun 19, 2004·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Vassil KaimaktchievChristopher L Corless
Oct 7, 2004·Cancer Research·Thomas BrabletzJean-Noel Freund
Nov 3, 2004·Cancer Research·Hiroyuki MutohKentaro Sugano
May 13, 2005·Annals of Oncology : Official Journal of the European Society for Medical Oncology·D CunninghamUNKNOWN ESMO Guidelines Task Force
Nov 30, 2005·Oncogene·L H DangD T Dang
Dec 7, 2005·Oncogene·L H DangD T Dang
Dec 20, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Matthew E WitekScott A Waldman
May 31, 2007·International Journal of Cancer. Journal International Du Cancer·MingZhou GuoJames G Herman
Jun 30, 2007·Oncogene·I GrossJ-N Freund
Oct 2, 2007·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Qiang LiuKhay Guan Yeoh
Apr 22, 2008·Cancer Science·Nobuhiro YamakawaTakeo Ohnishi
Feb 14, 2009·Journal of Gastroenterology and Hepatology·Rita A Busuttil, Alex Boussioutas
Mar 18, 2009·Cell Biology International·Yubo XieQiang Xiao
Apr 11, 2009·Oncology Reports·Yubo XieQiang Xiao
Jan 1, 2010·International Journal of Oncology·Yubo XieQiang Xiao
Jan 19, 2010·World Journal of Gastroenterology : WJG·Yao ZhengXiao-Ying Zhang
Jun 17, 2010·The Journal of Biological Chemistry·Mette BoydJesper T Troelsen
Aug 12, 2010·Cancer Research·Yuji TakakuraHideki Ohdan
Nov 4, 2010·Cancer Research·Jacqueline C ShultzCharles E Chalfant
Nov 18, 2010·Gastroenterology·Mary Ann S CrisseyJohn P Lynch
Dec 15, 2010·Gut·Rita BarrosRaquel Almeida
Citations
Feb 6, 2013·PloS One·Liduan ZhengQiangsong Tong
Jul 19, 2013·World Journal of Gastroenterology : WJG·Lin-Hai YanYu-Bo Xie
Feb 20, 2015·Molecular Medicine Reports·Li ZhuangQing Tian
Nov 28, 2012·Journal of Experimental & Clinical Cancer Research : CR·Xiao-Tong WangYu-Bo Xie
Jan 16, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Yasuyuki GotoNobuyuki Hamajima
Jul 28, 2016·Oncotarget·Zhan-Zhan LiLi-Zhang Chen
Aug 2, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Aledson Vitor FelipeNora Manoukian Forones
Jan 17, 2015·World Journal of Gastroenterology : WJG·Wei-Yuan WeiQiang Xiao
Jan 12, 2019·Cell Death & Disease·Junhui YuJianbao Zheng
Aug 6, 2015·Journal of Veterinary Science·Xuerui WanRun Wu
Dec 3, 2016·Oncology Letters·Yunshuai WangBaowei Han
Mar 1, 2021·BMC Gastroenterology·Dandan ChaiYang Xu
Apr 30, 2021·Genomics·Xingchen LiJianliu Wang