siRNA targeting of Cdx2 inhibits growth of human gastric cancer MGC-803 cells.

World Journal of Gastroenterology : WJG
Xiao-Tong WangQiang Xiao

Abstract

To investigate the effects of small interference RNA (siRNA) targeting of Cdx2 on human gastric cancer MGC-803 cells in vitro and in vivo. The recombinant pSilencer 4.1-Cdx2 siRNA plasmids were constructed and transfected into gastric cancer MGC-803 cells in vitro. The stable transfectants were selected. The effects of Cdx2 siRNA on growth, proliferation, cell cycle, apoptosis, migration and invasiveness of human gastric cancer MGC-803 cells were evaluated and the expression of phosphatase and tensin homolog (PTEN), caspase-9 and caspase-3 was observed in vitro by reverse transcription polymerase chain reaction (RT-PCR) and Western blotting analysis. We also investigated the effect of Cdx2 siRNA on growth of MGC-803 cells in nude mice in vivo. Cdx2 siRNA led to inhibition of endogenous Cdx2 mRNA and protein expression as determined by RT-PCR and Western blotting analysis. Cdx2 siRNA significantly inhibited cell growth and proliferation, blocked entry into the S-phase of the cell cycle, induced cell apoptosis, and reduced the motility and invasion of MGC-803 cells. Cdx2 siRNA also increased PTEN expression, and activated caspase-9 and caspase-3 in MGC-803 cells in vitro . In addition, siRNA targeting of Cdx2 inhibited the growth...Continue Reading

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Citations

Jul 19, 2013·World Journal of Gastroenterology : WJG·Lin-Hai YanYu-Bo Xie
Nov 28, 2012·Journal of Experimental & Clinical Cancer Research : CR·Xiao-Tong WangYu-Bo Xie
Jan 16, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Yasuyuki GotoNobuyuki Hamajima
Aug 2, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Aledson Vitor FelipeNora Manoukian Forones
Jan 17, 2015·World Journal of Gastroenterology : WJG·Wei-Yuan WeiQiang Xiao
Dec 3, 2016·Oncology Letters·Yunshuai WangBaowei Han
Mar 1, 2021·BMC Gastroenterology·Dandan ChaiYang Xu

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