siRNA Targeting the 2Apro Genomic Region Prevents Enterovirus 71 Replication In Vitro

PloS One
Haibing LiuJianguo Chen

Abstract

Enterovirus 71 (EV71) is the most important etiological agent of hand, foot, and mouth disease (HFMD) in young children, which is associated with severe neurological complications and has caused significant mortalities in recent HFMD outbreaks in Asia. However, there is no effective antiviral therapy against EV71. In this study, RNA interference (RNAi) was used as an antiviral strategy to inhibit EV71 replication. Three small interfering RNAs (siRNAs) targeting the 2Apro region of the EV71 genome were designed and synthesized. All the siRNAs were transfected individually into rhabdomyosarcoma (RD) cells, which were then infected with strain EV71-2006-52-9. The cytopathic effects (CPEs) in the infected RD cells, cell viability, viral titer, and viral RNA and protein expression were examined to evaluate the specific viral inhibition by the siRNAs. The results of cytopathogenicity and MTT tests indicated that the RD cells transfected with the three siRNAs showed slight CPEs and significantly high viability. The 50% tissue culture infective dose (TCID50) values demonstrated that the viral titer of the groups treated with three siRNAs were lower than those of the control groups. qRT-PCR and western blotting revealed that the levels ...Continue Reading

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Citations

Sep 5, 2019·Journal of Biomedical Science·Jing-Yi LinShin-Ru Shih
May 5, 2017·Frontiers in Cellular and Infection Microbiology·Xiaoxia YangHongbin Song
Oct 24, 2018·Frontiers in Microbiology·Yuefei JinGuangcai Duan
Aug 13, 2021·Expert Opinion on Drug Discovery·Chih-Yeu Fang, Chia-Chyi Liu

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Methods Mentioned

BETA
transfection
flow cytometry
Assay
inverted microscopy
PCR
ELISA

Software Mentioned

SPSS
BLAST

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