Sirolimus enhances the protection achieved by a DNA vaccine against Leishmania infantum.

Parasites & Vectors
Alba Martínez-FlórezAlhelí Rodríguez-Cortés

Abstract

Leishmaniases are a group of neglected tropical parasitic diseases, mainly affecting vulnerable populations of countries with poor socioeconomic status. Development of efficient vaccines is a priority due to the increasing incidence of drug resistance and toxicity to current treatments. In the search for a safe and efficient protective vaccine for human and dog visceral leishmaniases, we analyzed the suitability of the immunomodulatory drug sirolimus (SIR) to boost a preventive DNA vaccine against leishmaniasis. SIR is an already marketed drug that has been described to boost immune protection against different disease models and has also emerged as a promising therapeutic drug against L. major. Syrian hamsters were treated with SIR concomitantly with the administration of a DNA vaccine formulation consisting in four plasmids carrying the Leishmania genes LACK, TRYP, PAPLE22 and KMPII, respectively. Two weeks after the last vaccination, the animals were infected intraperitoneally with L. infantum parasites. Five weeks post-infection the parasite load was measured by real-time PCR in target tissues and immune response was evaluated by determining anti-Leishmania specific antibodies in combination with cytokine expression in the ...Continue Reading

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Datasets Mentioned

BETA
AF044679
PAPLE22
AF123892
U49695
X95267

Methods Mentioned

BETA
PCR
ELISA

Software Mentioned

WRS2
R
ENSEMBL
CLUSTALW
[UNK]
BLAST

Related Concepts

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