SIRT1 deacetylates SATB1 to facilitate MAR HS2-MAR ε interaction and promote ε-globin expression.

Nucleic Acids Research
Zheng XueChih-Chuan Liang

Abstract

The higher order chromatin structure has recently been revealed as a critical new layer of gene transcriptional control. Changes in higher order chromatin structures were shown to correlate with the availability of transcriptional factors and/or MAR (matrix attachment region) binding proteins, which tether genomic DNA to the nuclear matrix. How posttranslational modification to these protein organizers may affect higher order chromatin structure still pending experimental investigation. The type III histone deacetylase silent mating type information regulator 2, S. cerevisiae, homolog 1 (SIRT1) participates in many physiological processes through targeting both histone and transcriptional factors. We show that MAR binding protein SATB1, which mediates chromatin looping in cytokine, MHC-I and β-globin gene loci, as a new type of SIRT1 substrate. SIRT1 expression increased accompanying erythroid differentiation and the strengthening of β-globin cluster higher order chromatin structure, while knockdown of SIRT1 in erythroid k562 cells weakened the long-range interaction between two SATB1 binding sites in the β-globin locus, MAR(HS2) and MAR(ε). We also show that SIRT1 activity significantly affects ε-globin gene expression in a SA...Continue Reading

References

Oct 24, 2001·Cell·H VaziriR A Weinberg
Feb 16, 2002·Science·Job DekkerNancy Kleckner
Oct 11, 2002·Nature·Dag YasuiTerumi Kohwi-Shigematsu
Nov 1, 2002·Cell·Mariano Labrador, Victor G Corces
Apr 15, 2003·Nature Genetics·Shutao CaiTerumi Kohwi-Shigematsu
Jul 31, 2003·Molecular Cell·Marcella FulcoVittorio Sartorelli
Oct 5, 2007·Current Opinion in Genetics & Development·Sanjeev GalandeP Pavan Kumar
Dec 24, 2008·Molecular and Cellular Biology·Prabhat Kumar PurbeySanjeev Galande
Feb 4, 2009·Molecular and Cellular Biology·Natalia PediconiMassimo Levrero
Apr 1, 2009·Biochemical and Biophysical Research Communications·Huan GongChih-chuan Liang
Oct 7, 2010·Nucleic Acids Research·Katsunobu KashiwagiYasufumi Kaneda
Apr 21, 2011·Molecular Cell·Laia Bosch-PreseguéAlejandro Vaquero

❮ Previous
Next ❯

Citations

Jul 9, 2013·Bioscience, Biotechnology, and Biochemistry·Chieri Ida, Shin Ogata
Mar 26, 2015·Chemical Reviews·Hui Jing, Hening Lin
Aug 26, 2015·Biochimica Et Biophysica Acta·Ninu Poulose, Raghavan Raju
Apr 18, 2016·Experimental Cell Research·Mark T DuncanWilliam M Miller
May 9, 2016·The Journal of Nutritional Biochemistry·Estanislau NavarroHelmut Schröder
Jan 18, 2017·Medicinal Research Reviews·Matthias SchiedelManfred Jung
Aug 5, 2017·American Journal of Hematology·Yan DaiMartin H Steinberg
Mar 16, 2018·Frontiers in Molecular Neuroscience·Warren Winick-Ng, R Jane Rylett

❮ Previous
Next ❯

Methods Mentioned

BETA
acetylation
density gradient centrifugation
PCR
immunoprecipitation
ChIP
coIP
co-IP
genetic modifications
sumoylation

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.