Sirt1 exerts anti-inflammatory effects and promotes steroidogenesis in Leydig cells

Fertility and Sterility
Ling WuYun Feng

Abstract

To investigate the anti-inflammatory effects of Sirt1 and its roles in steroidogenesis in Leydig cells. In vitro cell culture study. Reproductive medical center. C57BL/6 male mice. TM3 Leydig cells were treated with proinflammatory cytokines including tumor necrosis factor-α, interleukin-6, or interleukin-1β. Sirt1 mRNA and protein levels were measured by real-time polymerase chain reaction and Western blotting in TM3 Leydig cells treated with proinflammatory cytokines. The cell viability was determined using MTT and BrdU incorporation assays. The effect of Sirt1 on the coactivation of steroidogenic factor 1 (SF-1) was characterized by coimmunoprecipitation and luciferase reporter assays. Sirt1 mRNA and protein levels were significantly down-regulated by proinflammatory cytokines treatment in TM3 Leydig cells. Sirt1 agonist or overexpression could efficiently protect cytotoxicity induced by proinflammatory cytokines. Sirt1 promoted steroidogenesis through its coactivation of SF-1. Sirt1 plays protective roles and promotes steroidogenesis in Leydig cells through its anti-inflammatory actions and coactivation of SF-1.

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Citations

Sep 15, 2015·Journal of Cellular Biochemistry·Balamurugan RamatchandirinChidambaram Prahalathan
Sep 8, 2015·The Journal of Allergy and Clinical Immunology·Mingyu LeeHyun-Woo Shin
Jun 7, 2015·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Mohanraj SadasivamChidambaram Prahalathan
Apr 29, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Mélodie DiotJoëlle Dupont
Mar 24, 2018·Reproductive Biology and Endocrinology : RB&E·Kristian Leisegang, Ralf Henkel
Jun 5, 2020·Reproduction in Domestic Animals = Zuchthygiene·Shi YinJian Li
Mar 20, 2021·Molecular and Cellular Biochemistry·Chithra LoganathanChidambaram Prahalathan
Apr 4, 2021·International Journal of Molecular Sciences·Fazal WahabRüdiger Behr
May 7, 2020·Protein and Peptide Letters·Sara ParrettiniGiovanni Luca

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