Abstract
The aim of the present study was to investigate the function and mechanism of sirtuin 1 (SIRT1) in spinal cord injury (SCI). Reverse transcription‑quantitative polymerase chain reaction was used to measure the expression levels of microRNA (miR)‑494. MTT assay, lactate dehydrogenase activity assay and flow cytometry were used to analyze the effects of miR‑494 on cell growth and apoptosis in a model of SCI. The present study demonstrated that SIRT1 expression was reduced; whereas miR‑494 expression was increased in a rat model of SCI. Overexpression of miR‑494 suppressed the protein expression levels of SIRT1, and induced p53 protein expression. Conversely, knockdown of miR‑494 induced SIRT1 protein expression in an in vitro model of SCI. Furthermore, overexpression of miR‑494 promoted cell apoptosis and decreased cell growth in an in vitro model of SCI; however, miR‑494 knockdown enhanced cell growth and inhibited cell apoptosis. Administration of a SIRT1 agonist reduced the effects of miR‑494 overexpression on cell apoptosis in an SCI model, whereas treatment with a p53 agonist reduced the effects of miR‑494 knockdown on cell apoptosis in an SCI model. Together, these findings suggested that SIRT1 may inhibit apoptosis of SCI ...Continue Reading
References
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 23, 2009·Alimentary Pharmacology & Therapeutics·H R AnchaM A Korsten
Nov 23, 2011·Spinal Cord·M L YangW Young
Jun 27, 2012·Archives of Physical Medicine and Rehabilitation·Brooke M WadsworthJennifer D Paratz
Nov 20, 2012·World Journal of Urology·Apostolos ApostolidisSherif Mourad
Jan 9, 2013·Spinal Cord·E C CelikE Lakse
Jul 19, 2013·Journal of Neurotrauma·Robert G GrossmanJefferson R Wilson
Sep 17, 2013·Archives of Physical Medicine and Rehabilitation·Rabih O DarouicheCharles G Minard
Apr 1, 2014·Journal of Neuroinflammation·Xiao-Qian LiHong Ma
May 16, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Marika ComegnaRaffaella Faraonio
Jun 3, 2014·Archives of Physical Medicine and Rehabilitation·Meegan G Van StraatenKristin D Zhao
Jan 13, 2015·Cytokine·Qiaoli LiangDaping Fan
May 13, 2015·Gene Therapy·Y LiuG Zhao
Jul 25, 2015·Virology Journal·Xin DengJian Liang
Aug 10, 2015·Archives of Physical Medicine and Rehabilitation·Dong-Il KimJustin Y Jeon
Oct 29, 2015·Journal of the American Heart Association·Daniela LobenweinJohannes Holfeld
Nov 28, 2015·Stem Cell Research & Therapy·Yongsun KimOh-Kyeong Kweon
May 26, 2016·Journal of Neuroinflammation·C M FreriaA L R Oliveira
Jan 6, 2017·Cell Death & Disease·Meng-Na ZhanQian Zhao
May 13, 2017·Redox Biology·Kristeena Ray WrightNalini Santanam
Citations
Feb 26, 2020·Bioscience Reports·Guang WanQiudong Liang
Jan 24, 2021·Molecular Neurobiology·Sheng FangZong-Sheng Yin
Jun 9, 2021·Neural Regeneration Research·Yan ZhouYan-Jie Jia