SIRT1 is a regulator in high glucose-induced inflammatory response in RAW264.7 cells

PloS One
Yanhui JiaDahai Hu

Abstract

Sepsis is defined as a systemic inflammatory response syndrome that disorders the functions of host immune system, including the imbalance between pro- and anti-inflammatory responses mediated by immune macrophages. Sepsis could also induce acute hyperglycemia. Studies have shown that the silent mating type information regulation 2 homolog 1 (SIRT1), an NAD+-dependent deacetylase, mediates NF-κb deacetylation and inhibits its function. Therefore, SIRT1 is likely to play an important role in high glucose-mediated inflammatory signalings. Here we demonstrate that high glucose significantly downregulates both the mRNA and protein levels of SIRT1 and upregulates the mRNA level and the release of two pro-inflammatory cytokines, IL-1β and TNF-α, in RAW264.7 macrophages. Interestingly, the reduced level of SIRT1 by high glucose is remarkably upregulated by SIRT1 activator SRT1720, while the level and the release of IL-1β and TNF-α significantly decrease with the use of SRT1720. However, when the function of SIRT1 is inhibited by EX527 or its expression is suppressed by RNAi, the upregulated level and release of IL-1β and TNF-α by high glucose are further increased. Taken together, these findings collectively suggest that SIRT1 is an i...Continue Reading

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Citations

Nov 1, 2017·Antioxidants & Redox Signaling·Patricia RadaÁngela M Valverde
Aug 5, 2020·Journal of Drug Targeting·Ye-Jee LeeSeung-Hyun Ko
Nov 11, 2015·Journal of Translational Medicine·Ling ZhouMing-Jun Wang
Jul 6, 2019·Journal of Diabetes and Metabolic Disorders·Aishwarya P DasareKiran Kalia
May 14, 2020·International Journal of Molecular Sciences·Cole LiechtyJunwang Xu
Nov 4, 2016·Nature Reviews. Drug Discovery·David F ToughRab K Prinjha
Jan 1, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alexandra L McCubbreyJeffrey L Curtis
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Apr 25, 2018·Journal of Leukocyte Biology·Allison RahtesLiwu Li
Dec 12, 2018·Critical Care Research and Practice·Lulan LiZhenhua Zeng

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Methods Mentioned

BETA
scraping
Protein Assay
ELISA
transfection
PCR

Software Mentioned

SPSS17
ImageJ

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